Sigel C W, Woolley J L, Nichol C A
J Pharm Sci. 1975 Jun;64(6):973-6. doi: 10.1002/jps.2600640617.
A spectrodensitometric method for the direct determination of sulfadiazine at the tissue residue level (0.1 ppm) is based upon the measurement of the fluorescence of a sulfadiazine-fluorescamine derivative formed directly on a TLC plate by dipping it into a fluorescamine solution. The linear dynamic range for the assay is about 150 from 200 to 0.2 ng, the lower limit of sensitivity. Recoveries from various spiked tissues including milk, eggs, liver, kidneys, muscle, skin, and fat varied with the tissue type but were reproducible. The assay technique has also been used for the assay of sulfamethoxazole and has been explored for use in specifically assaying sulfonamide mixtures.
一种用于直接测定组织残留水平（0.1 ppm）磺胺嘧啶的分光密度法，是基于将薄层层析板浸入荧光胺溶液中，直接在板上形成磺胺嘧啶-荧光胺衍生物的荧光测量。该测定的线性动态范围约为150，从200到0.2 ng，即灵敏度下限。从包括牛奶、鸡蛋、肝脏、肾脏、肌肉、皮肤和脂肪在内的各种加标组织中的回收率随组织类型而异，但具有可重复性。该测定技术也已用于磺胺甲恶唑的测定，并已探索用于特异性测定磺胺类混合物。
