de Torres C, Munell F, Ferrer I, Reventós J, Macaya A
Centre d'Investigacions en Bioquímica i Biologia Molecular Vall d'Hebron, Barcelona, Spain.
Neurosci Lett. 1997 Jul 11;230(1):1-4. doi: 10.1016/s0304-3940(97)00445-x.
The time course and localization of DNA fragmentation in a neonatal rat model of unilateral hypoxia-ischemia were assessed by means of the terminal transferase-mediated biotin dUTP nick end labeling (TUNEL) assay. TUNEL-positive cells were detected in the hemisphere ipsilateral to the ligation immediately after the injury and increased to reach a maximum 1-3 days later, then decreasing until day 10, in parallel with cell death identified by standard histological methods. Cells showing any of the different morphologies of chromatin condensation and fragmentation were labeled, particularly within the core of the ischemic lesion. These results, obtained in a paradigm of necrosis in the immature brain, add to previous evidence suggesting that some forms of non-apoptotic DNA fragmentation are labeled by the TUNEL assay.
采用末端脱氧核苷酸转移酶介导的生物素-dUTP缺口末端标记（TUNEL）法，评估新生大鼠单侧缺氧缺血模型中DNA片段化的时间进程和定位。损伤后立即在结扎同侧半球检测到TUNEL阳性细胞，1 - 3天后增加至最大值，然后减少直至第10天，这与标准组织学方法鉴定的细胞死亡情况平行。呈现染色质凝聚和片段化等任何不同形态的细胞都被标记，特别是在缺血性病变的核心区域。在未成熟脑坏死范例中获得的这些结果，补充了先前的证据，表明TUNEL法可标记某些形式的非凋亡性DNA片段化。
