用于固定人血栓调节蛋白的微波二氧化碳等离子体引发的丙烯酸在聚四氟乙烯上的气相接枝聚合反应

Microwave CO2 plasma-initiated vapour phase graft polymerization of acrylic acid onto polytetrafluoroethylene for immobilization of human thrombomodulin.

作者信息

Vasilets V N, Hermel G, König U, Werner C, Müller M, Simon F, Grundke K, Ikada Y, Jacobasch H J

机构信息

Institute for Energy Problems of Chemical Physics, Chernogolovka, Russia.

出版信息

Biomaterials. 1997 Sep;18(17):1139-45. doi: 10.1016/s0142-9612(97)00045-8.

DOI:10.1016/s0142-9612(97)00045-8

PMID:9259510

Abstract

The functionalization of polytetrafluoroethylene (PTFE) for human thrombomodulin (hTM) binding has been achieved by CO2 plasma activation and subsequent vapour phase graft polymerization of acrylic acid (AA). The PTFE surfaces after CO2 plasma treatment, AA grafting and hTM immobilization were characterized by attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy, X-ray photoelectron spectroscopy (XPS) and electron spin resonance (ESR) spectroscopy, as well as by zeta potential and wetting measurements to quantitatively control each step of modification. The activity of immobilized hTM was estimated by the protein C activation test.

摘要

通过二氧化碳等离子体活化以及随后的丙烯酸（AA）气相接枝聚合，实现了聚四氟乙烯（PTFE）与人血栓调节蛋白（hTM）结合的功能化。采用衰减全反射傅里叶变换红外光谱（ATR-FTIR）、X射线光电子能谱（XPS）和电子自旋共振（ESR）光谱，以及ζ电位和润湿性测量对经二氧化碳等离子体处理、AA接枝和hTM固定后的PTFE表面进行表征，以定量控制改性的每个步骤。通过蛋白C活化试验评估固定化hTM的活性。