编码大肠杆菌中CspA家族成员的cspD的生长阶段依赖性表达。
Growth-phase-dependent expression of cspD, encoding a member of the CspA family in Escherichia coli.
作者信息
Yamanaka K, Inouye M
机构信息
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway 08854, USA.
出版信息
J Bacteriol. 1997 Aug;179(16):5126-30. doi: 10.1128/jb.179.16.5126-5130.1997.
Abstract
The cspD gene of Escherichia coli encodes a protein of high sequence similarity with the cold shock protein CspA, but cspD expression is not induced by cold shock. In this study, we analyzed the regulation of cspD gene expression. By using a cspD-lacZ fusion and primer extension analysis, the expression of cspD was found to be dramatically induced by stationary-phase growth. However, this induction does not depend on the stationary-phase sigma factor sigmaS. Moreover, the expression of cspD is inversely dependent on growth rates and induced upon glucose starvation. Using a (p)ppGpp-depleted strain, we found that (p)ppGpp is one of the positive factors for the regulation of cspD expression.
摘要
大肠杆菌的cspD基因编码一种与冷休克蛋白CspA序列高度相似的蛋白质，但cspD的表达不会被冷休克诱导。在本研究中，我们分析了cspD基因表达的调控。通过使用cspD - lacZ融合和引物延伸分析，发现cspD的表达在稳定期生长时被显著诱导。然而，这种诱导并不依赖于稳定期的σ因子σS。此外，cspD的表达与生长速率呈负相关，并在葡萄糖饥饿时被诱导。使用一个（p）ppGpp缺失的菌株，我们发现（p）ppGpp是调控cspD表达的正调控因子之一。
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