大肠杆菌K-12基因组的一段718千碱基对的DNA序列，对应于连锁图谱上12.7 - 28.0分钟区域。

A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map.

作者信息

Oshima T, Aiba H, Baba T, Fujita K, Hayashi K, Honjo A, Ikemoto K, Inada T, Itoh T, Kajihara M, Kanai K, Kashimoto K, Kimura S, Kitagawa M, Makino K, Masuda S, Miki T, Mizobuchi K, Mori H, Motomura K, Nakamura Y, Nashimoto H, Nishio Y, Saito N, Horiuchi T

机构信息

Institute of Medical Science, University of Tokyo, Japan.

出版信息

DNA Res. 1996 Jun 30;3(3):137-55. doi: 10.1093/dnares/3.3.137.

DOI:10.1093/dnares/3.3.137

PMID:8905232

Abstract

The 718,122 base pair sequence of the Escherichia coli K-12 genome corresponding to the region from 12.7 to 28.0 minutes on the genetic map is described. This region contains at least 681 potential open reading frames, of which 277 (41%) have been previously identified, 147 (22%) are homologous to other known genes, 139 (20%) are identical or similar to the hypothetical genes registered in databases, and the remaining 118 (17%) do not show a significant similarity to any other gene. In this region, we assigned a cluster of cit genes encoding multienzyme citrate lyase, two clusters of fimbrial genes and a set of lysogenic phage genes encoding integrase, excisionase and repressor in the e14 genetic element. In addition, a new valine tRNA gene, designated valZ, and a family of long directly repeated sequences, LDR-A, -B and -C, were found.

摘要

描述了大肠杆菌K-12基因组718,122个碱基对序列，该序列对应于遗传图谱上12.7至28.0分钟的区域。该区域至少包含681个潜在的开放阅读框，其中277个（41%）先前已被鉴定，147个（22%）与其他已知基因同源，139个（20%）与数据库中登记的假设基因相同或相似，其余118个（17%）与任何其他基因均无显著相似性。在该区域，我们确定了一组编码多酶柠檬酸裂合酶的cit基因簇、两组菌毛基因以及一组在e14遗传元件中编码整合酶、切除酶和阻遏物的溶原性噬菌体基因。此外，还发现了一个新的缬氨酸tRNA基因，命名为valZ，以及一个长的直接重复序列家族，即LDR-A、-B和-C。