Matschke G H, Fagerstone K A
J Toxicol Environ Health. 1977 Oct;3(3):407-11. doi: 10.1080/15287397709529573.
Benzenesulfonic acid [(3-amino-2,4,6-trichlorophenyl)methylene] hydrazide, a candidate rodenticide coded as DRC-4575, was administered by gavage to pregnant female BALB/c mice. Each dose (5.5, 28.0, 42.0, 62.0 and 94.0 mg/kg) was given to one of five groups of ten mice on day 8 of gestation, making a total of five different dose groups on day 8. This same procedure was followed for days 9, 10, 11, 12, and 13. This made a total of 30 dose-day treatment groups. Six control females were dosed each day. Dam survival to day 18 declined as the dose increased; only 2% of the dams survived at 94 mg/kg. When the surviving females were killed at day 18, no significant differences were found between treatment and control animals in the number or weight of live fetuses, or in the ratio of male to female fetuses. However, the percentage of live fetuses was significantly lower and the number of resorptions was significantly higher for the treated dams at the 62 mg/kg dose level than for the control dams. Skeletal anomalies were limited primarily to unossified phalanges, which were probably related to the lower weights of those fetuses. Slight hydrocephalus occurred infrequently at all dose levels and in the controls, and was not dose-related. These data indicate that DRC-4575 would be embryotoxic only at doses of 62 mg/kg or higher and would not be teratogenic.
苯磺酸[(3-氨基-2,4,6-三氯苯基)亚甲基]酰肼，一种编码为DRC-4575的候选灭鼠剂，通过灌胃法给予怀孕的雌性BALB/c小鼠。在妊娠第8天，将每组10只小鼠中的一组给予每个剂量（5.5、28.0、42.0、62.0和94.0mg/kg），妊娠第8天共有五个不同剂量组。在第9、10、11、12和13天遵循相同的程序。这总共形成了30个剂量-日治疗组。每天给6只对照雌性小鼠给药。随着剂量增加，母鼠存活至第18天的比例下降；在94mg/kg剂量下，只有2%的母鼠存活。当在第18天处死存活的雌性小鼠时，在活胎数量或重量、或雌雄胎比例方面，治疗组和对照组动物之间未发现显著差异。然而，在62mg/kg剂量水平下，治疗组母鼠的活胎百分比显著较低，吸收胎数量显著较高。骨骼异常主要限于未骨化的指骨，这可能与这些胎儿体重较低有关。在所有剂量水平和对照组中，轻度脑积水偶尔出现，且与剂量无关。这些数据表明，DRC-4575仅在62mg/kg或更高剂量时具有胚胎毒性，不会致畸。
