Pfeffer A, Phegan M D, Bany J
AgResearch, Wallaceville Animal Research Centre, Upper Hutt, New Zealand.
Vet Immunol Immunopathol. 1997 Jul;57(3-4):315-25. doi: 10.1016/s0165-2427(97)00014-7.
The utility of a basophil histamine-release assay using washed whole blood cells was examined in lambs and was used to determine if homocytotropic antibody with specificity for Bovicola ovis was produced in response to infestation with the louse. Maximal histamine release in the assay in response to Concanavalin A, anti-ovine IgE monoclonal antibody and, in sensitized lambs, to B. ovis antigen ranged from 18 to 48%. Histamine release from blood cells in response to B. ovis antigen was significantly higher in louse-infested lambs than in louse-naive lambs and was significantly correlated with louse and cockle scores. Passive cutaneous anaphylaxis (PCA) tests were negative with sera obtained from the lambs at the same time as blood for the basophil histamine-release assay. Serum histamine levels also were significantly higher in the louse-infested lambs than in louse-naive lambs and were significantly correlated with louse and cockle scores. The present results support a role for B. ovis-specific homocytotropic antibody in the development of cockle and indicate that the basophil histamine-release assay is more sensitive than the PCA test.
利用洗涤过的全血细胞进行嗜碱性粒细胞组胺释放试验的效用在羔羊中进行了检测，并用于确定针对绵羊虱（Bovicola ovis）感染是否产生了具有特异性的亲同种细胞抗体。在该试验中，对刀豆球蛋白A、抗绵羊IgE单克隆抗体以及在致敏羔羊中对绵羊虱抗原的最大组胺释放率为18%至48%。感染虱子的羔羊血细胞对绵羊虱抗原的组胺释放率显著高于未感染虱子的羔羊，且与虱子和丘疹评分显著相关。在采集用于嗜碱性粒细胞组胺释放试验血液的同时，从羔羊获得的血清进行的被动皮肤过敏试验（PCA）为阴性。感染虱子的羔羊血清组胺水平也显著高于未感染虱子的羔羊，且与虱子和丘疹评分显著相关。目前的结果支持绵羊虱特异性亲同种细胞抗体在丘疹形成中的作用，并表明嗜碱性粒细胞组胺释放试验比PCA试验更敏感。
