Bailey D N
Department of Pathology, University of California, San Diego Medical Center 92103-8320, USA.
Ther Drug Monit. 1997 Aug;19(4):427-30. doi: 10.1097/00007691-199708000-00011.
The effect of amphetamine (AMPH), codeine (COD), methamphetamine (MEPH), morphine (MORP), and benzoylecgonine (BE) on the binding of cocaethylene (CE) and cocaine (COC) to human serum in vitro was investigated by equilibrium dialysis at 4 degrees C. Each compound was added individually at concentrations of 500, 1,000, or 2,000 nM to pooled human serum containing COC or CE at 500 nM concentration. For COC, the addition of COD, MEPH, and CE enhanced serum binding whereas MORP and BE decreased it. Variable effects on COC binding were noted for AMPH. For CE, the addition of COD and COC generally increased binding whereas MORP decreased it. No appreciable effect on CE binding was observed after adding AMPH, MEPH, and BE. Except for CE, AMPH, and MEPH in the presence of COC, the binding of COC and CE tended to be less with 2,000 nM of each added drug than at lower concentrations of them, presumably because of mass-action displacement of COC and CE at the higher concentration. These findings should be clinically important because these drugs are frequently found together in patients.
在4℃下通过平衡透析研究了苯丙胺（AMPH）、可待因（COD）、甲基苯丙胺（MEPH）、吗啡（MORP）和苯甲酰芽子碱（BE）对可卡因乙烯酯（CE）和可卡因（COC）与人血清体外结合的影响。将每种化合物分别以500、1000或2000 nM的浓度添加到含有500 nM浓度COC或CE的混合人血清中。对于COC，添加COD、MEPH和CE增强了血清结合，而MORP和BE则降低了血清结合。AMPH对COC结合有不同影响。对于CE，添加COD和COC通常增加结合，而MORP则降低结合。添加AMPH、MEPH和BE后，未观察到对CE结合有明显影响。除了在存在COC的情况下CE、AMPH和MEPH外，每种添加药物浓度为2000 nM时，COC和CE的结合往往比其较低浓度时更少，推测是因为在较高浓度下COC和CE的质量作用置换。这些发现应具有临床重要性，因为这些药物在患者中经常同时出现。
