Yi S Y, Hwang B K
Department of Agricultural Biology, Korea University, Seoul, Korea.
Mol Cells. 1997 Jun 30;7(3):408-13.
Inoculation of soybean (Glycine max L. cv. Jangyup) hypocotyls with Phytophthora sojae f. sp. glycines results in a marked accumulation of some pathogenesis-related (PR) proteins. A basic beta-1,3-glucanase (34 kDa) was purified from soybean hypocotyls infected by an incompatible race of P. sojae f. sp. glycines using CM-cellulose cation exchange chromatography and Bio-gel P-60 gel filtration. The purified soybean beta-1,3-glucanase cross-reacted with polyclonal antibody raised against a tomato beta-1,3-glucanase. The activity of beta-1,3-glucanase was much higher in the infected soybean hypocotyls than the healthy ones. The beta-1, 3-glucanase purified from soybean inhibited spore germination and hyphal growth of the chitin-negative fungus P. sojae f. sp. glycines, but did not show any antifungal activity against the chitin-containing fungi Alternaria mali, Colletotrichum gloeosporioides, and Magnaporthe grisea.
用大豆疫霉大豆专化型接种大豆（Glycine max L. cv. Jangyup）下胚轴会导致一些病程相关（PR）蛋白显著积累。使用CM - 纤维素阳离子交换色谱法和Bio - gel P - 60凝胶过滤法，从受大豆疫霉大豆专化型不亲和小种感染的大豆下胚轴中纯化出一种碱性β-1,3 - 葡聚糖酶（34 kDa）。纯化的大豆β-1,3 - 葡聚糖酶与针对番茄β-1,3 - 葡聚糖酶产生的多克隆抗体发生交叉反应。感染大豆下胚轴中β-1,3 - 葡聚糖酶的活性比健康下胚轴中的活性高得多。从大豆中纯化的β-1,3 - 葡聚糖酶抑制了几丁质阴性真菌大豆疫霉大豆专化型的孢子萌发和菌丝生长，但对含几丁质的真菌苹果链格孢、胶孢炭疽菌和稻瘟病菌没有任何抗真菌活性。
