Yi S Y, Hwang B K
Department of Agricultural Biology, Korea University, Seoul.
Mol Cells. 1998 Oct 31;8(5):556-64.
Differential display techniques were used to isolate cDNA clones corresponding to genes which were expressed in soybean hypocotyls by Phytophthora sojae f.sp. glycines infection. With a partial cDNA clone C20CI4 from the differential display PCR as a probe, a new basic peroxidase cDNA clone, designated GMIPER1, was isolated from a cDNA library of soybean hypocotyls infected with P. sojae f.sp. glycines. Sequence analysis revealed that the peroxidase clone encodes a mature protein of 35,813 Da with a putative signal peptide of 27 amino acids in its N-terminus. The amino acid sequence of the soybean peroxidase GMIPER1 is between 54-75% identical to other plant peroxidases including a soybean seed coat peroxidase. Southern blot analysis indicated that multiple copies of sequences related to GMIPER1 exist in the soybean genome. The mRNAs corresponding to the GMIPER1 cDNA accumulated predominantly in the soybean hypocotyls infected with the incompatible race of P. sojae f.sp. glycines, but were expressed at low levels in the compatible interaction. Soybean GMIPER1 mRNAs were not expressed in hypocotyls, leaves, stems, and roots of soybean seedlings. However, treatments with ethephon, salicylic acid or methyl jasmonate induced the accumulation of the GMIPER1 mRNAs in the different organs of soybean. These results suggest that the GMIPER1 gene encoding a putative pathogen-induced peroxidase may play an important role in induced resistance of soybean to P. sojae f.sp. glycines and in response to various external stresses.
采用差异显示技术分离与大豆疫霉大豆专化型感染大豆下胚轴中表达的基因相对应的cDNA克隆。以差异显示PCR获得的部分cDNA克隆C20CI4为探针，从大豆疫霉大豆专化型感染的大豆下胚轴cDNA文库中分离出一个新的碱性过氧化物酶cDNA克隆，命名为GMIPER1。序列分析表明，该过氧化物酶克隆编码一个成熟蛋白，分子量为35,813 Da，其N端有一个27个氨基酸的推定信号肽。大豆过氧化物酶GMIPER1的氨基酸序列与包括大豆种皮过氧化物酶在内的其他植物过氧化物酶的序列一致性为54 - 75%。Southern杂交分析表明，大豆基因组中存在多个与GMIPER1相关的序列拷贝。与GMIPER1 cDNA相对应的mRNA主要在被大豆疫霉大豆专化型非亲和小种感染的大豆下胚轴中积累，但在亲和互作中表达水平较低。大豆GMIPER1 mRNA在大豆幼苗的下胚轴、叶片、茎和根中均不表达。然而，乙烯利、水杨酸或茉莉酸甲酯处理可诱导GMIPER1 mRNA在大豆不同器官中积累。这些结果表明，编码推定的病原菌诱导过氧化物酶的GMIPER1基因可能在大豆对大豆疫霉大豆专化型的诱导抗性及对各种外界胁迫的响应中发挥重要作用。
