Ehret R, Heinkelein M, Siliciano R F, Jassoy C
Institute for Virology and Immunobiology, Julius-Maximilians University, Würzburg, Germany.
AIDS Res Hum Retroviruses. 1997 Aug 10;13(12):1017-21. doi: 10.1089/aid.1997.13.1017.
Human immunodeficiency virus (HIV) glycoprotein-specific CD4+ cytotoxic T lymphocytes (CTLs) lyse target cells in an MHC-restricted calcium-dependent fashion similar to the mechanism used by CD8+ CTLs. However, contact of unprimed peripheral blood CD4+ T cells with HIV glycoprotein-expressing cells has been shown to cause, in addition to cell-cell fusion, rapid cytolysis that may resemble antigen-specific cytotoxicity in the chromium release assay. In this study, the ability of glycoprotein-specific CD4+ CTLs to undergo similar fusion-related cytolysis was examined. The data obtained demonstrate that in addition to antigen-specific calcium-dependent cytotoxicity, envelope-specific CD4+ CTLs are involved in fusion-related, calcium-independent cytolysis.
人类免疫缺陷病毒（HIV）糖蛋白特异性CD4 + 细胞毒性T淋巴细胞（CTL）以与CD8 + CTL相同的MHC限制钙依赖方式裂解靶细胞。然而，已证明未致敏的外周血CD4 + T细胞与表达HIV糖蛋白的细胞接触，除了细胞间融合外，还会导致快速细胞溶解，这在铬释放试验中可能类似于抗原特异性细胞毒性。在本研究中，检测了糖蛋白特异性CD4 + CTL进行类似融合相关细胞溶解的能力。获得的数据表明，除了抗原特异性钙依赖细胞毒性外，包膜特异性CD4 + CTL还参与融合相关的、钙非依赖性细胞溶解。
