Coto-Montes A, Rodríguez-Colunga M J, Tolivia D, Sánchez-Barceló E
Department of Physiology and Pharmacology, School of Medicine, University of Cantabria, Santander, Spain.
Microsc Res Tech. 1997 Aug 1;38(3):311-4. doi: 10.1002/(SICI)1097-0029(19970801)38:3<311::AID-JEMT11>3.0.CO;2-Q.
The Harderian gland is a tubule-alveolar gland found within the orbit, on the posterior aspect of the eyeball. In mice, it is composed almost exclusively of secretory tubules and alveoli. The Harderian glands of transgenic mice, carrying the activated N-ras oncogene under the transcriptional control of the mouse mammary tumor virus long terminal repeat promoter (MMTV-LTR), were examined and compared to those of normal mice. Thirty transgenic mice provided by A. Pellicer (NYU) and 30 normal mice were examined in this study. Harderian glands were dissected, immersed in a formalin-based fixative, and embedded in paraffin. The sections of these glands were studied via histological techniques. Our results show that proliferative alterations in the Harderian glands of these transgenic mice are present even in the youngest animals. Such alterations correspond to different tumoral evolution stages, ranging from hyperplasia to wide tissue destruction. In the most advanced situations, these changes are accompanied by a glandular hypertrophy. Our results suggest a very high tumoral incidence in the Harderian glands of transgenic mice compared to normal mice. Tumors appear spontaneously in some areas, but not at the same time in the whole gland.
哈德氏腺是一种位于眼眶内眼球后部的小管泡状腺。在小鼠中，它几乎完全由分泌小管和腺泡组成。对携带在小鼠乳腺肿瘤病毒长末端重复启动子（MMTV-LTR）转录控制下的活化N-ras癌基因的转基因小鼠的哈德氏腺进行了检查，并与正常小鼠的哈德氏腺进行了比较。本研究检查了由A. Pellicer（纽约大学）提供的30只转基因小鼠和30只正常小鼠。解剖哈德氏腺，浸入基于福尔马林的固定剂中，然后石蜡包埋。通过组织学技术研究这些腺体的切片。我们的结果表明，这些转基因小鼠的哈德氏腺即使在最年幼的动物中也存在增殖性改变。这种改变对应于不同的肿瘤演变阶段，从增生到广泛的组织破坏。在最严重的情况下，这些变化伴有腺体肥大。我们的结果表明，与正常小鼠相比，转基因小鼠的哈德氏腺肿瘤发生率非常高。肿瘤在某些区域自发出现，但不是在整个腺体同时出现。
