Cowin G J, Leditschke I A, Crozier S, Brereton I M, Endre Z H
Department of Medicine, University of Queensland, Brisbane, Australia.
MAGMA. 1997 Jun;5(2):151-8. doi: 10.1007/BF02592246.
Volume-localized proton nuclear magnetic resonance spectroscopy was used as an assay of regional biochemistry in the isolated perfused rat kidney. This model eliminated artifacts caused by respiratory and cardiac motion experienced in vivo. Immersion of the kidney under its venous effluent reduced the susceptibility artifacts evoked by tissue-air interfaces. The rapid acquisition with relaxation enhancement imaging sequence was used for scout imaging. This gave excellent spatial resolution of the cortex, outer medulla, and inner medulla. Spectra were then acquired in 10 minutes using the volume-selective multipulse spectroscopy sequence from voxels with a volume of approximately 24 microL located within the cortical or medullary regions. Spectral peaks were assigned by the addition of known compounds to the perfusion medium and by comparison with spectra of protein-free extracts of cortex and medulla. The medullary region spectra were characterized by signals from the osmolytes betaine, glycerophosphorylcholine, and inositol. The spectra from the cortex were more complex and contained lesser contributions from osmolytes.
体积定位质子核磁共振波谱法被用作离体灌注大鼠肾脏区域生物化学的分析方法。该模型消除了体内呼吸和心脏运动引起的伪影。将肾脏浸入其静脉流出液下方可减少组织-空气界面引起的磁化率伪影。使用快速采集弛豫增强成像序列进行定位成像。这给出了皮质、外髓质和内髓质的出色空间分辨率。然后使用体积选择多脉冲波谱序列在10分钟内从位于皮质或髓质区域内、体积约为24微升的体素中采集波谱。通过向灌注介质中添加已知化合物并与皮质和髓质的无蛋白提取物的波谱进行比较来确定波谱峰。髓质区域波谱的特征是渗透压物质甜菜碱、甘油磷酸胆碱和肌醇的信号。皮质的波谱更复杂，渗透压物质的贡献较小。
