Esteban M A, Meseguer J
Department of Cell Biology, Faculty of Biology, University of Murcia, Spain.
Anat Rec. 1997 Aug;248(4):533-41. doi: 10.1002/(SICI)1097-0185(199708)248:4<533::AID-AR5>3.0.CO;2-M.
Although phagocytosis is considered the main nonspecific defense mechanism in fish, the significance of the influence of bacterial properties and assay conditions on this mechanism has not been established. We report the influence of leukocyte source, bacterial species, presence or absence of a bacterial wall, bacterial status (live or dead), and bacterial opsonization on in vitro phagocytosis by sea bass (Dicentrarchus labrax L.) macrophages.
Leukocytes were challenged with bacteria under different conditions and ultrastructurally studied. The number of phagocytic macrophages and the number of bacteria ingested per macrophage were quantified by microscopical counting, and the data obtained were statistically analyzed following general linear models.
Macrophages from sea bass peritoneal exudate showed a greater capacity to engulf bacteria than did those isolated from blood, which, in turn, had greater engulfment properties than did those isolated from head-kidney. Greater phagocytic activity was observed in the assays carried out with Escherichia coli than in those carried out with Salmonella typhimurium, especially when the TA1950 S. typhimurium strain (with wall) was used. Macrophages showed greater phagocytic activity against live bacteria. Bacterial opsonization had a variable effect on phagocytosis. Sea bass macrophages did not always ingest the same number of bacteria.
Sea bass macrophages have strong bacterial phagocytic activity. Significant differences were found that had been influenced by bacterial properties as well as by assay conditions. Positive correlation between the number of phagocytic macrophages and the number of bacteria ingested per phagocyte was observed.
尽管吞噬作用被认为是鱼类主要的非特异性防御机制，但细菌特性和检测条件对该机制影响的重要性尚未明确。我们报道了白细胞来源、细菌种类、细菌细胞壁的有无、细菌状态（活的或死的）以及细菌调理作用对海鲈（Dicentrarchus labrax L.）巨噬细胞体外吞噬作用的影响。
在不同条件下用细菌刺激白细胞并进行超微结构研究。通过显微镜计数对吞噬性巨噬细胞的数量和每个巨噬细胞摄取的细菌数量进行定量，并按照一般线性模型对获得的数据进行统计分析。
海鲈腹膜渗出液中的巨噬细胞比从血液中分离的巨噬细胞具有更强的吞噬细菌能力，而从血液中分离的巨噬细胞又比从头肾中分离的巨噬细胞具有更强的吞噬特性。用大肠杆菌进行的检测中观察到的吞噬活性比用鼠伤寒沙门氏菌进行的检测中更高，特别是当使用TA1950鼠伤寒沙门氏菌菌株（有细胞壁）时。巨噬细胞对活细菌表现出更强的吞噬活性。细菌调理作用对吞噬作用有不同影响。海鲈巨噬细胞摄取的细菌数量并不总是相同。
海鲈巨噬细胞具有很强的细菌吞噬活性。发现了受细菌特性以及检测条件影响的显著差异。观察到吞噬性巨噬细胞数量与每个吞噬细胞摄取的细菌数量之间存在正相关。
