Maier R, Glatz A, Mosbacher J, Bilbe G
Novartis Pharma Inc., Basel, CH-4002, Switzerland.
Biochem Biophys Res Commun. 1997 Aug 18;237(2):297-302. doi: 10.1006/bbrc.1997.7135.
Cellular responses to ATP/UTP and analogs are mediated by G-protein coupled P2Y receptors and have been proposed to play a role in the regulation of bone metabolism. Using a degenerate PCR approach on MG-63 cell cDNA we found PCR fragments coding for human P2Y1 and a new receptor, P2Y6. cDNA cloning of the P2Y6 receptor identified three cDNA isoforms. Two contained the same contiguous ORFs but differed in their 5' UTRs and may therefore originate by alternative splicing whereas the third represents a pseudogene. Analysis of P2Y receptor subtype expression in human bone and the osteoblastic cell lines OHS-4 and MG-63 by RT-PCR showed that all known human P2Y receptor subtypes (P2Y1, P2Y2, P2Y4, P2Y6, and P2Y7) were expressed. In contrast, analysis of brain-derived cell lines suggests that a selective expression of P2Y receptor subtypes occurs in brain tissue.
细胞对ATP/UTP及其类似物的反应由G蛋白偶联的P2Y受体介导，并且有人提出这些反应在骨代谢调节中发挥作用。我们采用简并PCR方法对MG-63细胞cDNA进行研究，发现了编码人P2Y1和一种新受体P2Y6的PCR片段。P2Y6受体的cDNA克隆鉴定出三种cDNA异构体。其中两种含有相同的连续开放阅读框，但5'非翻译区不同，因此可能通过可变剪接产生，而第三种代表假基因。通过逆转录聚合酶链反应（RT-PCR）分析人骨及成骨细胞系OHS-4和MG-63中P2Y受体亚型的表达，结果显示所有已知的人P2Y受体亚型（P2Y1、P2Y2、P2Y4、P2Y6和P2Y7）均有表达。相比之下，对脑源性细胞系的分析表明，P2Y受体亚型在脑组织中存在选择性表达。
