Hermann D M, Luppi P H, Peyron C, Hinckel P, Jouvet M
Physiologisches Institut, Fachbereich Humanmedizin, Justus-Liebig-Universität, Giessen, Germany.
J Chem Neuroanat. 1997 Jun;13(1):1-21. doi: 10.1016/s0891-0618(97)00019-7.
The aim of the present study was to identify the specific afferent projections to the rostral and caudal nucleus raphe magnus, the gigantocellular reticular nucleus pars alpha and the rostral nucleus raphe pallidus. For this purpose, small iontophoretic injections of the sensitive retrograde tracer choleratoxin (subunit b) were made in each of these structures. In agreement with previous retrograde studies, after all injection sites, a substantial to large number of labeled neurons were observed in the dorsal hypothalamic area and dorsolateral and ventrolateral parts of the periaqueductal gray, and a small to moderate number were found in the lateral preoptic area, bed nucleus of the stria terminalis, paraventricular hypothalamic nucleus, central nucleus of the amygdala, lateral hypothalamic area, parafascicular area, parabrachial nuclei, subcoeruleus area and parvocellular reticular nucleus. In addition, depending on the nucleus injected, we observed a variable number of retrogradely labeled cells in other regions. After injections in the rostral nucleus raphe magnus, a large number of labeled cells were seen in the prelimbic, infralimbic, medial and lateral precentral cortices and the dorsal part of the periaqueductal gray. In contrast, after injections in the other nuclei, fewer cells were localized in these structures. Following raphe pallidus injections, a substantial to large number of labeled cells were observed in the medial preoptic area, median preoptic nucleus, ventromedial part of the periaqueductal gray, Kölliker-Fuse and lateral paragigantocellular reticular nuclei. Following injections in the other areas, a small to moderate number of cells appeared. After gigantocellular reticular pars alpha injections, a very large and substantial number of labeled neurons were found in the deep mesencephalic reticular formation and oral pontine reticular nucleus, respectively. After the other injections, fewer cells were seen. Following rostral raphe magnus or raphe pallidus injections, a substantial number of labeled cells were observed in the insular and perirhinal cortices. Following caudal raphe magnus or gigantocellular reticular pars alpha injections, fewer cells were found. After raphe magnus or gigantocellular reticular pars alpha injections, a moderate to substantial number of cells were localized in the fields of Forel, lateral habenular nucleus and ventral caudal pontine reticular nucleus. Following raphe pallidus injections, only a small number of cells were seen. Our data indicate that the rostral and caudal parts of the nucleus raphe magnus, the gigantocellular reticular nucleus pars alpha and the nucleus raphe pallidus receive afferents of comparable strength from a large number of structures. In addition, a number of other afferents give rise to stronger inputs to one or two of the four nuclei studied. Such differential inputs might be directed to populations of neurons with different physiological roles previously recorded specifically in these nuclei.
本研究的目的是确定向吻侧和尾侧中缝大核、巨细胞网状核α部以及吻侧中缝苍白核的特定传入投射。为此，在这些结构中的每一个中进行了小剂量离子导入敏感逆行示踪剂霍乱毒素（亚基b）的注射。与先前的逆行研究一致，在所有注射部位之后，在下丘脑背侧区以及导水管周围灰质的背外侧和腹外侧部分观察到大量至极大量的标记神经元，在视前外侧区、终纹床核、下丘脑室旁核、杏仁核中央核、下丘脑外侧区、束旁核、臂旁核、蓝斑下区和小细胞网状核中发现少量至中等数量的标记神经元。此外，根据注射的核团不同，我们在其他区域观察到数量不等的逆行标记细胞。在吻侧中缝大核注射后，在前边缘皮质、边缘下皮质、中央前回内侧和外侧皮质以及导水管周围灰质背侧部分可见大量标记细胞。相比之下，在其他核团注射后，这些结构中的标记细胞较少。在中缝苍白核注射后，在视前内侧区、视前正中核、导水管周围灰质腹内侧部分、 Kölliker-Fuse核和外侧巨细胞旁网状核中观察到大量至极大量的标记细胞。在其他区域注射后，出现少量至中等数量的细胞。在巨细胞网状核α部注射后，分别在中脑深部网状结构和脑桥嘴侧网状核中发现极大量和大量的标记神经元。在其他注射后，可见的细胞较少。在吻侧中缝大核或中缝苍白核注射后，在岛叶皮质和梨状周围皮质中观察到大量标记细胞。在尾侧中缝大核或巨细胞网状核α部注射后，发现的细胞较少。在中缝大核或巨细胞网状核α部注射后，在Forel区、外侧缰核和脑桥尾侧腹侧网状核中定位到中等数量至大量的细胞。在中缝苍白核注射后，仅可见少量细胞。我们的数据表明，中缝大核的吻侧和尾侧部分、巨细胞网状核α部和中缝苍白核从大量结构接收强度相当的传入纤维。此外，一些其他传入纤维对所研究的四个核团中的一两个产生更强的输入。这种差异输入可能针对先前在这些核团中特异性记录的具有不同生理作用的神经元群体。
