Forebrain projections of the rostral nucleus raphe magnus shown by iontophoretic application of choleratoxin b in rats.

The nucleus raphe magnus belongs to the thermoafferent system. Following iontophoretic choleratoxin b injections in its rostral part, a substantial to large number of anterogradely labeled varicose fibres were observed in the medial and lateral preoptic areas, the bed nucleus, the substantia innominata, the ventral pallidum, the median preoptic nucleus, the paraventricular hypothalamic nucleus, the central amygdaloid nucleus and the lateral and dorsal hypothalamic areas. A small to moderate number were seen in the septal nuclei, the diagonal band, the magnocellular preoptic nucleus, the anterior hypothalamic area and the paraventricular and intralaminar thalamic nuclei. After choleratoxin b injections in the preoptic, dorsal and lateral hypothalamic areas, a substantial number of retrogradely labeled serotonin immunonegative neurones were specifically found in the rostral nucleus raphe magnus. Thus, non-serotonergic rostral nucleus raphe magnus cells might directly modulate hypothalamic thermointegrative neurones.