小鼠基因组中16种新的驱动蛋白超家族（KIF）蛋白的鉴定与分类。
Identification and classification of 16 new kinesin superfamily (KIF) proteins in mouse genome.
作者信息
Nakagawa T, Tanaka Y, Matsuoka E, Kondo S, Okada Y, Noda Y, Kanai Y, Hirokawa N
机构信息
Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113, Japan.
出版信息
Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9654-9. doi: 10.1073/pnas.94.18.9654.
KIF (kinesin superfamily) proteins are microtubule-dependent molecular motors that play important roles in intracellular transport and cell division. The extent to which KIFs are involved in various transporting phenomena, as well as their regulation mechanism, are unknown. The identification of 16 new KIFs in this report doubles the existing number of KIFs known in the mouse. Conserved nucleotide sequences in the motor domain were amplified by PCR using cDNAs of mouse nervous tissue, kidney, and small intestine as templates. The new KIFs were studied with respect to their expression patterns in different tissues, chromosomal location, and molecular evolution. Our results suggest that (i) there is no apparent tendency among related subclasses of KIFs of cosegregation in chromosomal mapping, and (ii) according to their tissue distribution patterns, KIFs can be divided into two classes-i.e., ubiquitous and specific tissue-dominant. Further characterization of KIFs may elucidate unknown fundamental phenomena underlying intracellular transport. Finally, we propose a straightforward nomenclature system for the members of the mouse kinesin superfamily.
驱动蛋白超家族（KIF）蛋白是依赖微管的分子马达，在细胞内运输和细胞分裂中发挥重要作用。KIF参与各种运输现象的程度及其调控机制尚不清楚。本报告中鉴定出的16种新的KIF使小鼠中已知的KIF数量增加了一倍。使用小鼠神经组织、肾脏和小肠的cDNA作为模板，通过PCR扩增马达结构域中的保守核苷酸序列。对新的KIF在不同组织中的表达模式、染色体定位和分子进化进行了研究。我们的结果表明：（i）在染色体图谱中，KIF相关亚类之间没有明显的共分离趋势；（ii）根据其组织分布模式，KIF可分为两类，即普遍存在的和特定组织占主导的。对KIF的进一步表征可能会阐明细胞内运输背后未知的基本现象。最后，我们为小鼠驱动蛋白超家族的成员提出了一个简单的命名系统。
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