Kawasaki M, Satow Y, Ohya Y, Anraku Y
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan.
FEBS Lett. 1997 Aug 4;412(3):518-20. doi: 10.1016/s0014-5793(97)00850-8.
Protein splicing is an autocatalytic reaction of a single polypeptide in which a spliced intervening sequence is excised out and the two external regions are ligated with the peptide bond to yield two mature proteins. We examined the reaction mechanism using a folding-dependent in vitro protein splicing system. Protein splicing proceeds at an optimal pH of 7 and is an intramolecular reaction. The reaction is not inhibited by potential protease inhibitors, suggesting that its mechanism is different from those catalyzed by known proteases.
蛋白质剪接是单个多肽的自催化反应，其中被剪接的插入序列被切除，两个外部区域通过肽键连接以产生两种成熟蛋白质。我们使用依赖折叠的体外蛋白质剪接系统研究了反应机制。蛋白质剪接在最适pH值7下进行，是一种分子内反应。该反应不受潜在蛋白酶抑制剂的抑制，这表明其机制与已知蛋白酶催化的机制不同。
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