归巢内切核酸酶PI-sceI的蛋白质剪接结构域负责特异性DNA结合。
The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding.
作者信息
Grindl W, Wende W, Pingoud V, Pingoud A
机构信息
Institut für Biochemie, Justus-Liebig-Universität, Heinrich-Buff-Ring 58, D-35392 Giessen, Germany.
出版信息
Nucleic Acids Res. 1998 Apr 15;26(8):1857-62. doi: 10.1093/nar/26.8.1857.
Abstract
The homing endonuclease PI- Sce I consists of a protein splicing domain (I) and an endonucleolytic domain (II). To characterize the two domains with respect to their contribution to DNA recognition we cloned, purified and characterized the isolated domains. Both domains have no detectable endonucleolytic activity. Domain I binds specifically to the PI- Sce I recognition sequence, whereas domain II displays only weak non-specific DNA binding. In the specific complex with domain I the DNA is bent to a similar extent as observed with the initial complex formed between PI- Sce I and DNA. Our results indicate that protein splicing domain I is also involved in recognition of the DNA substrate.
摘要
归巢核酸内切酶PI-Sce I由一个蛋白质剪接结构域（I）和一个核酸内切酶结构域（II）组成。为了表征这两个结构域对DNA识别的贡献，我们克隆、纯化并表征了分离出的结构域。两个结构域均未检测到核酸内切酶活性。结构域I特异性结合PI-Sce I识别序列，而结构域II仅表现出微弱的非特异性DNA结合。在与结构域I形成的特异性复合物中，DNA的弯曲程度与PI-Sce I和DNA形成的初始复合物中观察到的相似。我们的结果表明，蛋白质剪接结构域I也参与了DNA底物的识别。
相似文献
1
The protein splicing domain of the homing endonuclease PI-sceI is responsible for specific DNA binding.归巢内切核酸酶PI-sceI的蛋白质剪接结构域负责特异性DNA结合。
Nucleic Acids Res. 1998 Apr 15;26(8):1857-62. doi: 10.1093/nar/26.8.1857.
2
High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI.酿酒酵母归巢内切酶PI-SceI结构域I的高分辨率晶体结构。
Nucleic Acids Res. 2002 Sep 15;30(18):3962-71. doi: 10.1093/nar/gkf523.
3
Structural and functional analysis of the homing endonuclease PI-sceI by limited proteolytic cleavage and molecular cloning of partial digestion products.通过有限蛋白酶解和部分消化产物的分子克隆对归巢内切酶PI-sceI进行结构和功能分析。
Biochemistry. 1998 Jun 2;37(22):8233-43. doi: 10.1021/bi980013d.
4
Mapping of a DNA binding region of the PI-sceI homing endonuclease by affinity cleavage and alanine-scanning mutagenesis.通过亲和切割和丙氨酸扫描诱变对PI-sceI归巢内切酶的DNA结合区域进行定位。
Biochemistry. 1999 Sep 28;38(39):12621-8. doi: 10.1021/bi991192h.
5
Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence.内含肽归巢内切酶PI-SceI与其识别序列结合的晶体结构。
Nat Struct Biol. 2002 Oct;9(10):764-70. doi: 10.1038/nsb840.
6
The monomeric homing endonuclease PI-SceI has two catalytic centres for cleavage of the two strands of its DNA substrate.单体归巢内切酶PI-SceI有两个催化中心，用于切割其DNA底物的两条链。
EMBO J. 1999 Dec 15;18(24):6908-16. doi: 10.1093/emboj/18.24.6908.
7
A model for the PI-SceIxDNA complex based on multiple base and phosphate backbone-specific photocross-links.基于多个碱基和磷酸主链特异性光交联的PI-SceIxDNA复合物模型。
J Mol Biol. 2000 Jul 21;300(4):867-75. doi: 10.1006/jmbi.2000.3872.
8
Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity.PI-SceI的晶体结构，一种具有蛋白质剪接活性的归巢内切酶。
Cell. 1997 May 16;89(4):555-64. doi: 10.1016/s0092-8674(00)80237-8.
9
Substrate recognition and induced DNA distortion by the PI-SceI endonuclease, an enzyme generated by protein splicing.PI-SceI核酸内切酶对底物的识别及诱导的DNA扭曲，PI-SceI核酸内切酶是一种通过蛋白质剪接产生的酶。
J Mol Biol. 1996 Oct 25;263(2):163-80. doi: 10.1006/jmbi.1996.0567.
10
Probing the structure of the PI-SceI-DNA complex by affinity cleavage and affinity photocross-linking.通过亲和切割和亲和光交联探究PI-SceI-DNA复合物的结构。
J Biol Chem. 2000 Jan 28;275(4):2705-12. doi: 10.1074/jbc.275.4.2705.
引用本文的文献
1
Mitochondrial genome diversity of revealed by a fatal case of granulomatous amoebic encephalitis.一例肉芽肿性阿米巴脑炎致死病例所揭示的线粒体基因组多样性。
Front Microbiol. 2023 May 5;14:1162963. doi: 10.3389/fmicb.2023.1162963. eCollection 2023.
2
Structural Basis for the Propagation of Homing Endonuclease-Associated Inteins.归巢内切核酸酶相关内含肽传播的结构基础。
Front Mol Biosci. 2022 Mar 16;9:855511. doi: 10.3389/fmolb.2022.855511. eCollection 2022.
3
Protein Splicing Activity of the PolB-c Intein Is Sensitive to Homing Endonuclease Domain Mutations.PolB-c 内含肽的蛋白剪接活性对归巢内切酶结构域突变敏感。
Biochemistry. 2020 Sep 15;59(36):3359-3367. doi: 10.1021/acs.biochem.0c00512. Epub 2020 Sep 2.
4
Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.Meganucleases 和其他靶向基因组工程工具：基因治疗的观点和挑战。
Curr Gene Ther. 2011 Feb;11(1):11-27. doi: 10.2174/156652311794520111.
5
Evolution of I-SceI homing endonucleases with increased DNA recognition site specificity.提高 DNA 识别位点特异性的 I-SceI 归巢内切酶的进化。
J Mol Biol. 2011 Jan 7;405(1):185-200. doi: 10.1016/j.jmb.2010.10.029. Epub 2010 Oct 26.
6
Mutational analysis of active-site residues in the Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease: Asp(122) and Asp(193) are crucial to the double-stranded DNA cleavage activity whereas Asp(218) is not.对麻风分枝杆菌 RecA 内含肽活性位点残基的突变分析，一种 LAGLIDADG 类内含肽的归巢内切核酸酶：Asp(122)和 Asp(193)对双链 DNA 切割活性至关重要，而 Asp(218)则不是。
Protein Sci. 2010 Jan;19(1):111-23. doi: 10.1002/pro.292.
7
Homology modeling and mutational analysis of Ho endonuclease of yeast.酵母Ho核酸内切酶的同源建模与突变分析
Genetics. 2004 Feb;166(2):721-8. doi: 10.1534/genetics.166.2.721.
8
High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI.酿酒酵母归巢内切酶PI-SceI结构域I的高分辨率晶体结构。
Nucleic Acids Res. 2002 Sep 15;30(18):3962-71. doi: 10.1093/nar/gkf523.
9
Degeneration of a homing endonuclease and its target sequence in a wild yeast strain.野生酵母菌株中归巢内切酶及其靶序列的退化
Nucleic Acids Res. 2001 Oct 15;29(20):4215-23. doi: 10.1093/nar/29.20.4215.
10
Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility.归巢内切酶：对内含子/内含肽移动性催化剂的结构与功能洞察
Nucleic Acids Res. 2001 Sep 15;29(18):3757-74. doi: 10.1093/nar/29.18.3757.
本文引用的文献
1
Modular organization of inteins and C-terminal autocatalytic domains.内含肽和C端自催化结构域的模块化组织。
Protein Sci. 1998 Jan;7(1):64-71. doi: 10.1002/pro.5560070106.
2
Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing.来自偶发分枝杆菌的GyrA内含肽的晶体结构揭示了蛋白质剪接的结构基础。
Nat Struct Biol. 1998 Jan;5(1):31-6. doi: 10.1038/nsb0198-31.
3
Statistical modeling and analysis of the LAGLIDADG family of site-specific endonucleases and identification of an intein that encodes a site-specific endonuclease of the HNH family.LAGLIDADG家族位点特异性内切核酸酶的统计建模与分析以及对编码HNH家族位点特异性内切核酸酶的内含肽的鉴定。
Nucleic Acids Res. 1997 Nov 15;25(22):4626-38. doi: 10.1093/nar/25.22.4626.
4
Crystal structure of a Hedgehog autoprocessing domain: homology between Hedgehog and self-splicing proteins.刺猬蛋白自切割结构域的晶体结构：刺猬蛋白与自我剪接蛋白之间的同源性
Cell. 1997 Oct 3;91(1):85-97. doi: 10.1016/s0092-8674(01)80011-8.
5
Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution.蛋白质剪接结构域的遗传学定义：功能性小内含肽支持结构预测及内含肽进化模型
Proc Natl Acad Sci U S A. 1997 Oct 14;94(21):11466-71. doi: 10.1073/pnas.94.21.11466.
6
Protein splicing in the yeast Vma1 protozyme: evidence for an intramolecular reaction.酵母Vma1前体酶中的蛋白质剪接：分子内反应的证据。
FEBS Lett. 1997 Aug 4;412(3):518-20. doi: 10.1016/s0014-5793(97)00850-8.
7
Homing endonucleases: keeping the house in order.归巢内切酶：维持秩序
Nucleic Acids Res. 1997 Sep 1;25(17):3379-88. doi: 10.1093/nar/25.17.3379.
8
Statistical modeling, phylogenetic analysis and structure prediction of a protein splicing domain common to inteins and hedgehog proteins.内含肽和刺猬蛋白共有的蛋白质剪接结构域的统计建模、系统发育分析及结构预测
J Comput Biol. 1997 Summer;4(2):193-214. doi: 10.1089/cmb.1997.4.193.
9
Mapping metal ions at the catalytic centres of two intron-encoded endonucleases.定位两种内含子编码的内切核酸酶催化中心的金属离子
EMBO J. 1997 Jun 2;16(11):3272-81. doi: 10.1093/emboj/16.11.3272.
10
Identification of three core regions essential for protein splicing of the yeast Vma1 protozyme. A random mutagenesis study of the entire Vma1-derived endonuclease sequence.酵母Vma1前体酶蛋白剪接所必需的三个核心区域的鉴定。对整个Vma1来源的内切核酸酶序列的随机诱变研究。
J Biol Chem. 1997 Jun 20;272(25):15668-74. doi: 10.1074/jbc.272.25.15668.
Zotero 插件