Payne P W, Sebo T J, Doudkine A, Garner D, MacAulay C, Lam S, LeRichie J C, Palcic B
Department of Pathology, Faculty of Medicine, University of British Columbia, Vancouver, Canada.
Mayo Clin Proc. 1997 Aug;72(8):697-704. doi: 10.4065/72.8.697.
To investigate the hypothesis that image cytometry of sputum specimens can detect squamous carcinoma without requiring visually abnormal cells.
The sensitivity and specificity of image cytometry were evaluated in a case-control study.
Seventy-three sputum slides from the Mayo portion of the National Cancer Institute Cooperative Early Lung Cancer Study were restained by a modified Feulgen method. We examined 40 slides from 9 patients in whom squamous carcinoma developed and 33 slides from 11 patients in whom no cancer developed during a follow-up of at least 5 years. Images of normal epithelial nuclei were collected by using an automated image cytometer. Discriminant analysis was used to determine differences in DNA distribution of normal nuclei in sputum specimens from noncancer patients versus normal nuclei in sputum samples from patients in whom carcinoma developed.
By using features based on DNA distribution, 74% correct classification of nuclei was possible without human review of the material and without the use of visually abnormal nuclei. A receiver operating characteristic curve demonstrated sensitivities and specificities, including 40% sensitivity and 90% specificity.
Although this study was limited to 20-year-old slides and squamous cell carcinoma, automated image cytometry detected a substantial proportion of patients with squamous cell cancer without using visually abnormal nuclei.
探讨痰液标本图像细胞术可在无需可见异常细胞的情况下检测鳞状细胞癌的假说。
在一项病例对照研究中评估图像细胞术的敏感性和特异性。
对来自美国国立癌症研究所合作早期肺癌研究梅奥部分的73张痰涂片采用改良福尔根法重新染色。我们检查了来自9例发生鳞状细胞癌患者的40张涂片以及来自11例在至少5年随访期间未发生癌症患者的33张涂片。使用自动图像细胞仪收集正常上皮细胞核的图像。采用判别分析来确定非癌症患者痰液标本中正常细胞核与发生癌症患者痰液标本中正常细胞核的DNA分布差异。
基于DNA分布特征，无需人工查看材料且不使用可见异常细胞核，细胞核分类的正确率可达74%。一条受试者工作特征曲线显示出敏感性和特异性，包括40%的敏感性和90%的特异性。
尽管本研究仅限于20年之久的涂片及鳞状细胞癌，但自动图像细胞术在不使用可见异常细胞核的情况下检测出了相当比例的鳞状细胞癌患者。
