Jung H G, Mertens D R, Payne A J
USDA-Agricultural Research Service, St. Paul, MN 55108, USA.
J Dairy Sci. 1997 Aug;80(8):1622-8. doi: 10.3168/jds.S0022-0302(97)76093-4.
The acid detergent lignin and Klason lignin methods were compared for their correlation with forage digestibility. Thirty-six forages, including C3 legumes and C3 and C4 grasses, were analyzed for sulfuric acid detergent lignin, Klason lignin, and in vitro digestibilities of dry matter (DM) and neutral detergent fiber (NDF). Twenty of these forages were also fed to lambs at restricted intake for measurement of DM and NDF digestibilities. Lignin concentrations determined by the two lignin methods were positively correlated, and the Klason lignin value was always greater than the acid detergent lignin concentration. The largest differences were observed for grass forages. Digestibilities of forage DM and NDF were negatively correlated with both lignin methods for the in vitro system and the lamb digestibility trials. The degree of correlation for the two lignin methods with digestibility was generally similar across all forages and within forage classes. Slopes of linear regressions of digestibility on lignin concentration did not differ between legumes and grasses. Although the sulfuric acid detergent lignin and Klason lignin procedures gave very different estimates of forage lignin concentration, they were similarly correlated with digestibility and should yield predictions of forage digestibility that have similar accuracy.
比较了酸性洗涤木质素法和克拉斯森木质素法与饲料消化率的相关性。对36种饲料进行了分析，包括C3豆科植物以及C3和C4禾本科植物，测定了其硫酸酸性洗涤木质素、克拉斯森木质素以及干物质（DM）和中性洗涤纤维（NDF）的体外消化率。还以限食方式将其中20种饲料喂给羔羊，以测定DM和NDF的消化率。通过两种木质素法测定的木质素浓度呈正相关，且克拉斯森木质素值始终大于酸性洗涤木质素浓度。禾本科饲料的差异最为明显。在体外系统和羔羊消化率试验中，饲料DM和NDF的消化率与两种木质素法均呈负相关。两种木质素法与消化率的相关程度在所有饲料以及各类饲料中总体相似。豆类和禾本科植物中，消化率对木质素浓度的线性回归斜率没有差异。虽然硫酸酸性洗涤木质素法和克拉斯森木质素法对饲料木质素浓度的估计差异很大，但它们与消化率的相关性相似，并且应该能得出准确性相似的饲料消化率预测结果。
