Bamba T, Matsui R, Watabe K, Mayumi T
Pharmaceutical Research Center, Shiseido Company, Limited, Yokohama, Japan.
PDA J Pharm Sci Technol. 1997 Jul-Aug;51(4):156-60.
n-alkylpolyoxyethylene surfactants (CnH2n+1O(CH2CH2O)mH; CnEm) showed a strong enhancing effect on the inactivation of lipopolysaccharide (LPS) by heat treatment over a wide range of temperatures. The effect of CnE8 (n = 10-16) was observed above the critical micelle concentration (CMC) and above the cloud point, and was influenced by the length of the alkyl chains. The efficacy of the surfactants was in the order C10E8 < C12E8, C16E8 < C14E8. However, the hydrophilic moiety seemed to have no influence. An 80-95% solution of n-butanol showed a similar effect, indicating that LPS was more effectively inactivated in the oily phase of the surfactants than in water. The effect of surfactant on the hydrodynamic diameter of LPS was the same before and after steam-heat treatment for 20 minutes at 121 degrees C. Each surfactant disaggregated LPS without alteration of the activity of LPS before the heat treatment. We consider that the surfactants interact with LPS in the region of lipid A in a manner that favors loss of the activities of LPS during heating.
正烷基聚氧乙烯表面活性剂（CnH2n+1O(CH2CH2O)mH；CnEm）在很宽的温度范围内对通过热处理使脂多糖（LPS）失活表现出强烈的增强作用。在临界胶束浓度（CMC）以上和浊点以上观察到CnE8（n = 10 - 16）的作用，并且受烷基链长度的影响。表面活性剂的效力顺序为C10E8 < C12E8，C16E8 < C14E8。然而，亲水部分似乎没有影响。正丁醇的80 - 95%溶液表现出类似的效果，表明LPS在表面活性剂的油相中比在水中更有效地失活。在121℃蒸汽热处理20分钟前后，表面活性剂对LPS流体动力学直径的影响相同。每种表面活性剂在热处理前使LPS解聚而不改变LPS的活性。我们认为表面活性剂以有利于加热过程中LPS活性丧失的方式在脂质A区域与LPS相互作用。
