Diallinas G, Pateraki I, Sanmartin M, Scossa A, Stilianou E, Panopoulos N J, Kanellis A K
Institute of Viticulture, Vegetable Crops & Floriculture, National Agriculture Research Foundation, Heraklion, Crete, Greece.
Plant Mol Biol. 1997 Jul;34(5):759-70. doi: 10.1023/a:1005851527227.
A small family of at least four genes encoding melon ascorbate oxidase (AO) has been identified and three members of it have been cloned. Preliminary DNA sequence determination suggested that melon AO genes code for enzymes homologous to ascorbate oxidases from other plants and similar to other multicopper oxidases. We describe detailed molecular studies addressing melon AO expression during organ specific differentiation, fruit development and ripening, and in response to wounding. In particular, AO transcript accumulation was induced in ovaries and the outer mesocarp of mature preclimacteric melon fruits, before the expression of genes encoding the necessary enzymatic activities for ethylene biosynthesis. On the other hand, AO was not expressed in late stages of fruit ripening and was repressed in wounded fruits. The role of ethylene in transcriptional regulation of AO is discussed.
已鉴定出一个至少由四个编码甜瓜抗坏血酸氧化酶（AO）的基因组成的小家族，并克隆了其中三个成员。初步的DNA序列测定表明，甜瓜AO基因编码的酶与其他植物的抗坏血酸氧化酶同源，且与其他多铜氧化酶相似。我们描述了针对甜瓜AO在器官特异性分化、果实发育和成熟以及对伤口响应过程中表达的详细分子研究。特别是，在乙烯生物合成所需酶活性编码基因表达之前，AO转录本积累在成熟前跃变期甜瓜果实的子房和外中果皮中被诱导。另一方面，AO在果实成熟后期不表达，在受伤果实中受到抑制。本文还讨论了乙烯在AO转录调控中的作用。
