Koyama M, Kurotaki H, Yagihashi N, Aizawa S, Sugai M, Kamata Y, Oyama T, Yagihashi S
Department of Pathology, Hirosaki University School of Medicine, Japan.
Histopathology. 1997 Aug;31(2):134-9. doi: 10.1046/j.1365-2559.1997.2100842.x.
Two cases of adenomyoepithelioma of the breast were examined by immunohistochemistry to evaluate proliferative activity of epithelial and myoepithelial components.
The tumours showed a bicellular pattern of gland-forming epithelial cells and proliferative myoepithelial cells with clear cytoplasm. They showed foci of monotonous growth of myoepithelial cells devoid of glands with low mitotic rate (1-2/10 high-power fields) and mild cytological atypia. Immunohistochemically, the glandular cells were positive for epithelial membrane antigen, cytokeratin (KL-1 and CAM5.2) and carcinoembryonic antigen, whereas tumour cells with clear cytoplasm were reactive with muscle-specific actin (MSA), alpha smooth muscle actin, vimentin, and S100 protein but negative for desmin. Proliferative activities assessed by MIB-1 (Ki-67)/MSA positive cell index were greater in myoepithelial cells in both cases (19.2% and 17.7%) as compared to those in epithelial cells (MIB-1/CAM5.2 index: 10.2% and 9.5%).
These results might account for the previous findings that myoepithelial components predominate over the epithelial ones in an advanced stage of this tumour as well as in recurrent or metastatic lesions.
对两例乳腺腺肌上皮瘤进行免疫组织化学检查，以评估上皮和肌上皮成分的增殖活性。
肿瘤呈现出由形成腺管的上皮细胞和具有透明细胞质的增殖性肌上皮细胞组成的双细胞模式。它们显示出肌上皮细胞单调生长的病灶，无腺管形成，有丝分裂率低（1 - 2/10个高倍视野），细胞学异型性轻微。免疫组织化学方面，腺细胞上皮膜抗原、细胞角蛋白（KL - 1和CAM5.2）及癌胚抗原呈阳性，而具有透明细胞质的肿瘤细胞对肌特异性肌动蛋白（MSA）、α平滑肌肌动蛋白、波形蛋白和S100蛋白呈反应性，但结蛋白呈阴性。通过MIB - 1（Ki - 67）/MSA阳性细胞指数评估的增殖活性，在两例中肌上皮细胞均高于上皮细胞（分别为19.2%和17.7%，而上皮细胞的MIB - 1/CAM5.2指数分别为10.2%和9.5%）。
这些结果可能解释了先前的发现，即在该肿瘤的晚期以及复发或转移病灶中，肌上皮成分比上皮成分占优势。
