Toth C A, Nasir M A, Ho C L, Mittra R A
Department of Ophthalmology, Duke University Medical Center, Durham, North Carolina, USA.
Retina. 1997;17(4):315-20. doi: 10.1097/00006982-199707000-00007.
To test the efficacy of a single intraocular injection of various doses of recombinant desulphatohirudin variant 1 (Revasc) in a rabbit model after lensectomy-vitrectomy surgery.
Standard fragmatome lensectomies and core vitrectomies were performed on Dutch Belted rabbits. In a masked fashion, 17 control eyes received single intraocular injections of lactated Ringer's solution, and the treated eyes, with eight eyes per treatment group, received single intraocular injections of hirudin at doses of 0.02 microgram, 2 micrograms, 10 micrograms, 50 micrograms, or 200 micrograms.
Intraocular injections of hirudin were effective in preventing postoperative fibrin formation at doses of 2 micrograms. There was no notable intraoperative bleeding. With an injection of 2.0 micrograms hirudin there was no hemorrhage at any time after surgery; with an injection of 200 micrograms hirudin, however, there was notable bleeding.
Hirudin is effective in the prevention of postoperative fibrin formation with a single intraocular injection of 2.0 micrograms after lensectomy-vitrectomy surgery in the rabbit model. There was no bleeding at the effective dose of 2.0 micrograms; at 100 times the effective dose, however, there was significant postoperative bleeding.
在兔模型中，测试晶状体切除联合玻璃体切除术后单次眼内注射不同剂量重组去硫酸水蛭素变体1（瑞伐生）的疗效。
对荷兰带兔进行标准晶状体破碎术和核心玻璃体切除术。以盲法，17只对照眼接受单次眼内注射乳酸林格液，治疗组的眼，每组8只，分别接受单次眼内注射剂量为0.02微克、2微克、10微克、50微克或200微克的水蛭素。
眼内注射水蛭素剂量为2微克时可有效预防术后纤维蛋白形成。术中无明显出血。注射2.0微克水蛭素后术后任何时间均无出血；然而，注射200微克水蛭素后有明显出血。
在兔模型中，晶状体切除联合玻璃体切除术后单次眼内注射2.0微克水蛭素可有效预防术后纤维蛋白形成。有效剂量2.0微克时无出血；然而，有效剂量的100倍时术后有明显出血。
