Vais H, Williamson M S, Hick C A, Eldursi N, Devonshire A L, Usherwood P N
Department of Life Science, University of Nottingham, UK.
FEBS Lett. 1997 Aug 18;413(2):327-32. doi: 10.1016/s0014-5793(97)00931-9.
Pyrethroid insensitivity in resistant (kdr) insects has been correlated with a leucine to phenylalanine replacement in the S6 transmembrane segment of domain II of the axonal sodium channel alpha(para)-subunit. An alpha-subunit of rat brain type II sodium channel containing this mutation has been expressed and its sensitivity to permethrin compared with that of the wild-type channel. The steady-state activation curve of the mutant was shifted 14 mV in the depolarizing direction. We propose that an equivalent shift of the sodium current activation curve in kdr insects could account for their low sensitivity to permethrin toxicity.
抗性（kdr）昆虫对拟除虫菊酯的不敏感性与轴突钠通道α（para）亚基结构域II的S6跨膜片段中亮氨酸替换为苯丙氨酸有关。已表达了含有该突变的大鼠脑II型钠通道的α亚基，并将其对氯菊酯的敏感性与野生型通道进行了比较。突变体的稳态激活曲线在去极化方向上移动了14 mV。我们认为，kdr昆虫中钠电流激活曲线的等效移动可以解释它们对氯菊酯毒性的低敏感性。
