Meyer M F, Kreil G, Aschauer H
Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.
FEBS Lett. 1997 Aug 18;413(2):385-8. doi: 10.1016/s0014-5793(97)00936-8.
The membrane-bound PH-20 hyaluronidase is known to be essential for fertilization. Here we addressed the question whether the soluble hyaluronidase from bull teste is related to the PH-20 polypeptide. The sequence of the membrane-bound PH-20 hyaluronidase from bovine sperm was determined via cDNA cloning. In parallel, from a commercial preparation of bovine hyaluronidase the major 60-kDa form was purified to apparent homogeneity. The soluble enzyme was digested with two different proteases and with cyanogen bromide and the amino acid sequence of 44 different fragments was determined. All the peptide sequences could be aligned to the sequence deduced from the cloned cDNAs. Our results thus show that the soluble 60-kDa hyaluronidase from bovine testes is a glycoprotein derived from the sperm PH-20 enzyme. As compared to the primary translation product of the PH-20 mRNA, it lacks the signal peptide at the amino terminus and 56 amino acids at the carboxyl end. These results demonstrate that the soluble 60-kDa enzyme is a fragment of the PH-20 hyaluronidase. It is currently not known whether the soluble testes hyaluronidase has a distinct biological function.
已知膜结合型PH - 20透明质酸酶对受精至关重要。在此，我们探讨了来自公牛睾丸的可溶性透明质酸酶是否与PH - 20多肽相关的问题。通过cDNA克隆确定了牛精子膜结合型PH - 20透明质酸酶的序列。同时，从牛透明质酸酶的商业制剂中纯化出主要的60 kDa形式，使其达到明显的均一性。用两种不同的蛋白酶和溴化氰消化可溶性酶，并测定了44个不同片段的氨基酸序列。所有肽序列都可以与从克隆的cDNA推导的序列比对。因此，我们的结果表明，来自牛睾丸的可溶性60 kDa透明质酸酶是一种源自精子PH - 20酶的糖蛋白。与PH - 20 mRNA的初级翻译产物相比，它在氨基末端缺少信号肽，在羧基末端缺少56个氨基酸。这些结果表明，可溶性60 kDa酶是PH - 20透明质酸酶的一个片段。目前尚不清楚可溶性睾丸透明质酸酶是否具有独特的生物学功能。
