Zeiler T A, Kretschmer V
Department of Transfusion Medicine and Hemostaseology, Philipps University Clinics Marburg, Germany.
Transfusion. 1997 Aug;37(8):791-7. doi: 10.1046/j.1537-2995.1997.37897424400.x.
Automated collection of blood components with a cell separator (MCS 3p, Haemonetics), was performed according to three protocols.
The first protocol provided 2 units of fresh-frozen plasma (FFP); and one buffy coat-poor red cell (RBC) concentrate in additive solution. The second protocol included an additional in-line filtration of the RBC in a closed system after storage at 4 degrees C for 24 hours. In the third protocol, an additional platelet concentrate (PC) was recovered from the buffy coat. Cell counts and biochemical characterization of the RBCs (n = 20 each) were determined on Days 0, 1, 14, 28, and 49.
The RBC volume was 336 +/- 9 mL (first protocol), 337 +/- 7 mL (second protocol) and 293 +/- 12 mL (third protocol) with a hematocrit of 59 +/- 2, 53 +/- 3, and 61 +/- 5, percent respectively. On Day 49, hemolysis was 0.24 +/- 0.1 percent (first protocol), 0.33 +/- 0.32 percent (second protocol), and 0.38 +/- 0.1 percent (third protocol). The filtered RBC concentrate met the international standards for white cell-reduced RBCs. Filtration resulted in a clinically irrelevant increase of hemolysis. The in vitro RBC values (lactate dehydrogenase, 2-hydroxybutyrate dehydrogenase, hemolysis, potassium, 2,3 DPG, ATP) were at least equal to those in RBCs collected by conventional whole-blood donation. There is a trend toward extended preservation of 2,3 DPG in RBCs collected by apheresis. Two units of FFP could be collected with each donation (first protocol: 420 +/- 55 mL, 5.4 +/- 7 WBCs/microL, 6.5 +/- 5 x 10(3) platelets/microL; second protocol: 440 +/- 33 mL, 3 +/- 5.2 WBCs/microL, 32 +/- 12 x 10(3) platelets/microL; third protocol: 398 +/- 32 mL, 5 +/- 12 WBCs/microL; 3.4 +/- 3.5 x 10(3) platelets/microL). PCs prepared from the buffy coat collected by the third protocol contained 90 +/- 30 x 10(9) platelets in 88 +/- 14 mL of plasma. In vitro test results in these PCs were superior to those in PCs collected by conventional whole-blood donation. The procedure was well tolerated by all donors. No adverse reactions appeared.
Erythroplasmapheresis with the MCS 3p cell separator is a useful alternative to conventional whole-blood donation and separation.
使用血细胞分离机（MCS 3p，Haemonetics公司）按照三种方案自动采集血液成分。
第一种方案采集2单位新鲜冰冻血浆（FFP）和1单位添加保存液的少白细胞红细胞（RBC）浓缩液。第二种方案包括在4℃储存24小时后，在封闭系统中对红细胞进行额外的在线过滤。第三种方案是从富含血小板血浆中回收额外的血小板浓缩液（PC）。在第0、1、14、28和49天对红细胞（每组n = 20）进行细胞计数和生化特性分析。
红细胞体积在第一种方案中为336±9 mL，第二种方案中为337±7 mL，第三种方案中为293±12 mL，血细胞比容分别为59±2%、53±3%和61±5%。在第49天，溶血率在第一种方案中为0.24±0.1%，第二种方案中为0.33±0.32%，第三种方案中为0.38±0.1%。过滤后的红细胞浓缩液符合白细胞去除红细胞的国际标准。过滤导致溶血率有临床无关紧要的增加。体外红细胞值（乳酸脱氢酶、2 - 羟基丁酸脱氢酶、溶血、钾、2,3 - 二磷酸甘油酸、三磷酸腺苷）至少与传统全血捐献采集的红细胞相当。通过单采术采集的红细胞中2,3 - 二磷酸甘油酸有延长保存的趋势。每次捐献可采集2单位FFP（第一种方案：420±55 mL，5.4±7个白细胞/微升，6.5±5×10³个血小板/微升；第二种方案：440±33 mL，3±5.2个白细胞/微升，32±12×10³个血小板/微升；第三种方案：398±32 mL，5±12个白细胞/微升，3.4±3.5×10³个血小板/微升）。通过第三种方案采集的富含血小板血浆制备的PC在88±14 mL血浆中含有90±30×10⁹个血小板。这些PC的体外检测结果优于传统全血捐献采集的PC。所有捐献者对该操作耐受性良好。未出现不良反应。
使用MCS 3p血细胞分离机进行红细胞单采血浆术是传统全血捐献和分离的一种有用替代方法。
