使用MCS 3p细胞分离器进行自动化血液成分采集：三种制备低白细胞层和白细胞去除红细胞及血浆方案的评估

Automated blood component collection with the MCS 3p cell separator: evaluation of three protocols for buffy coat-poor and white cell-reduced packed red cells and plasma.

作者信息

Zeiler T A, Kretschmer V

机构信息

Department of Transfusion Medicine and Hemostaseology, Philipps University Clinics Marburg, Germany.

出版信息

Transfusion. 1997 Aug;37(8):791-7. doi: 10.1046/j.1537-2995.1997.37897424400.x.

DOI:10.1046/j.1537-2995.1997.37897424400.x

PMID:9280322

Abstract

BACKGROUND

Automated collection of blood components with a cell separator (MCS 3p, Haemonetics), was performed according to three protocols.

STUDY DESIGN AND METHODS

The first protocol provided 2 units of fresh-frozen plasma (FFP); and one buffy coat-poor red cell (RBC) concentrate in additive solution. The second protocol included an additional in-line filtration of the RBC in a closed system after storage at 4 degrees C for 24 hours. In the third protocol, an additional platelet concentrate (PC) was recovered from the buffy coat. Cell counts and biochemical characterization of the RBCs (n = 20 each) were determined on Days 0, 1, 14, 28, and 49.

RESULTS

The RBC volume was 336 +/- 9 mL (first protocol), 337 +/- 7 mL (second protocol) and 293 +/- 12 mL (third protocol) with a hematocrit of 59 +/- 2, 53 +/- 3, and 61 +/- 5, percent respectively. On Day 49, hemolysis was 0.24 +/- 0.1 percent (first protocol), 0.33 +/- 0.32 percent (second protocol), and 0.38 +/- 0.1 percent (third protocol). The filtered RBC concentrate met the international standards for white cell-reduced RBCs. Filtration resulted in a clinically irrelevant increase of hemolysis. The in vitro RBC values (lactate dehydrogenase, 2-hydroxybutyrate dehydrogenase, hemolysis, potassium, 2,3 DPG, ATP) were at least equal to those in RBCs collected by conventional whole-blood donation. There is a trend toward extended preservation of 2,3 DPG in RBCs collected by apheresis. Two units of FFP could be collected with each donation (first protocol: 420 +/- 55 mL, 5.4 +/- 7 WBCs/microL, 6.5 +/- 5 x 10(3) platelets/microL; second protocol: 440 +/- 33 mL, 3 +/- 5.2 WBCs/microL, 32 +/- 12 x 10(3) platelets/microL; third protocol: 398 +/- 32 mL, 5 +/- 12 WBCs/microL; 3.4 +/- 3.5 x 10(3) platelets/microL). PCs prepared from the buffy coat collected by the third protocol contained 90 +/- 30 x 10(9) platelets in 88 +/- 14 mL of plasma. In vitro test results in these PCs were superior to those in PCs collected by conventional whole-blood donation. The procedure was well tolerated by all donors. No adverse reactions appeared.

CONCLUSION

Erythroplasmapheresis with the MCS 3p cell separator is a useful alternative to conventional whole-blood donation and separation.

摘要

背景

使用血细胞分离机（MCS 3p，Haemonetics公司）按照三种方案自动采集血液成分。

研究设计与方法

第一种方案采集2单位新鲜冰冻血浆（FFP）和1单位添加保存液的少白细胞红细胞（RBC）浓缩液。第二种方案包括在4℃储存24小时后，在封闭系统中对红细胞进行额外的在线过滤。第三种方案是从富含血小板血浆中回收额外的血小板浓缩液（PC）。在第0、1、14、28和49天对红细胞（每组n = 20）进行细胞计数和生化特性分析。

结果

红细胞体积在第一种方案中为336±9 mL，第二种方案中为337±7 mL，第三种方案中为293±12 mL，血细胞比容分别为59±2%、53±3%和61±5%。在第49天，溶血率在第一种方案中为0.24±0.1%，第二种方案中为0.33±0.32%，第三种方案中为0.38±0.1%。过滤后的红细胞浓缩液符合白细胞去除红细胞的国际标准。过滤导致溶血率有临床无关紧要的增加。体外红细胞值（乳酸脱氢酶、2 - 羟基丁酸脱氢酶、溶血、钾、2,3 - 二磷酸甘油酸、三磷酸腺苷）至少与传统全血捐献采集的红细胞相当。通过单采术采集的红细胞中2,3 - 二磷酸甘油酸有延长保存的趋势。每次捐献可采集2单位FFP（第一种方案：420±55 mL，5.4±7个白细胞/微升，6.5±5×10³个血小板/微升；第二种方案：440±33 mL，3±5.2个白细胞/微升，32±12×10³个血小板/微升；第三种方案：398±32 mL，5±12个白细胞/微升，3.4±3.5×10³个血小板/微升）。通过第三种方案采集的富含血小板血浆制备的PC在88±14 mL血浆中含有90±30×10⁹个血小板。这些PC的体外检测结果优于传统全血捐献采集的PC。所有捐献者对该操作耐受性良好。未出现不良反应。