Sonntag J, Stiller B, Walka M M, Maier R F
Department of Neonatology, Virchow Clinic, Humboldt University, and the German Heart Institute, Berlin.
Transfusion. 1997 Aug;37(8):798-803. doi: 10.1046/j.1537-2995.1997.37897424401.x.
Fresh-frozen plasma (FFP) is widely used in patients with coagulation disorders and simultaneous complement activation. Complement activation in FFP itself is poorly investigated.
The concentration of anaphylatoxins C3a and C5a, the complement precursors C1q and factor B, and complement function were measured in 40 consecutively administered FFP units in two pediatric neonatal intensive care units. In 12 samples, the measurements were also performed after incubation with inulin.
In 15 of 40 FFP units, both anaphylatoxin concentrations were below the upper cutoff levels reported for healthy humans (C3a, 500 microg/L; C5a, 5 microg/L). Anaphylatoxin levels were higher in FFP units produced by apheresis than in those from blood donation. Complement activation of FFP by inulin increased anaphylatoxin concentration, whereas C1q and factor B levels, and complement function remained unchanged.
Elevated concentrations of anaphylatoxin are frequently found in FFP units produced by apheresis. Studies are necessary to investigate the reasons for complement activation and the possibilities of prevention during apheresis. As the concentrations of complement precursors and complement function did not change with activation in FFP, these studies should include measurement of the anaphylatoxins C3a and C5a.
新鲜冷冻血浆（FFP）广泛应用于凝血障碍及同时伴有补体激活的患者。FFP本身的补体激活情况鲜少被研究。
在两个儿科新生儿重症监护病房，对连续输注的40个FFP单位中的过敏毒素C3a和C5a浓度、补体前体C1q和B因子以及补体功能进行了测量。在12个样本中，还在与菊粉孵育后进行了测量。
在40个FFP单位中的15个中，两种过敏毒素浓度均低于健康人报告的上限水平（C3a，500微克/升；C5a，5微克/升）。单采血浆术制备的FFP单位中的过敏毒素水平高于献血制备的FFP单位。菊粉对FFP的补体激活增加了过敏毒素浓度，而C1q和B因子水平以及补体功能保持不变。
单采血浆术制备的FFP单位中经常发现过敏毒素浓度升高。有必要开展研究以调查补体激活的原因以及单采血浆术期间的预防可能性。由于FFP中补体前体浓度和补体功能在激活时未发生变化，这些研究应包括测量过敏毒素C3a和C5a。
