Sonntag J, Emeis M, Vornwald A, Strauss E, Maier R F
Department of Neonatology, Charité-Virchow-Medical Center, Humboldt University, Berlin, Germany.
Transfus Med. 1998 Sep;8(3):205-8. doi: 10.1046/j.1365-3148.1998.00150.x.
Elevated anaphylatoxin concentrations have been found in fresh frozen plasma packs produced by apheresis. The aim of this study was to investigate anaphylatoxin generation during apheresis production in relation to two frequently used techniques: employing either centrifugation alone or centrifugation with simultaneous filtration. The concentrations of C3a. C5a and sC5b-9 were measured in 50 plasmas after apheresis and before freezing generated by combined centrifugation and filtration and in 50 plasmas generated solely by centrifugation and in the corresponding 100 donors before apheresis. The median C3a concentration increased during apheresis by centrifugation alone from 62 (donor) to 380 micrograms L-1 (FFP pack) and during apheresis by combined centrifugation and filtration from 70 to 992 micrograms L-1. The median C5a concentration increased during apheresis by centrifugation alone from 0.38 to 0.83 microgram L-1 and during apheresis by combined centrifugation and filtration from 0.29 to 4.9 micrograms L-1. The soluble terminal complement complex increased only by combined centrifugation and filtration apheresis from 162 to 426 micrograms L-1. Complement activation during apheresis by combined centrifugation and filtration is more pronounced than that obtained by the centrifugation method alone. Changes in the construction or the materials used in the membrane-type separation units may reveal possibilities of prevention.
在单采制备的新鲜冰冻血浆袋中发现过敏毒素浓度升高。本研究的目的是调查单采制备过程中与两种常用技术相关的过敏毒素生成情况：单独采用离心法或离心同时进行过滤。在50份经离心和过滤联合处理后、冷冻前的单采血浆以及50份仅通过离心法制备的血浆中，以及在相应的100名献血者单采前，测量了C3a、C5a和sC5b-9的浓度。仅通过离心法进行单采时，C3a浓度中位数从62（献血者）升高至380微克/升（新鲜冰冻血浆袋），而通过离心和过滤联合进行单采时，C3a浓度中位数从70升高至992微克/升。仅通过离心法进行单采时，C5a浓度中位数从0.38升高至0.83微克/升，而通过离心和过滤联合进行单采时，C5a浓度中位数从0.29升高至4.9微克/升。可溶性末端补体复合物仅在通过离心和过滤联合进行单采时从162微克/升升高至426微克/升。通过离心和过滤联合进行单采时补体激活比单独采用离心法更明显。膜式分离装置的结构或所用材料的改变可能揭示预防的可能性。
