Hosoda F, Arai Y, Kitamura E, Inazawa J, Fukushima M, Tokino T, Nakamura Y, Jones C, Kakazu N, Abe T, Ohki M
Radiobiology Division, National Cancer Centre Research Institute, Tokyo, Japan.
Genes Cells. 1997 May;2(5):345-57. doi: 10.1046/j.1365-2443.1997.1250324.x.
Human chromosome 11 is one of the autosomes on which many disease genes have been mapped. Many different types of map, including a radiation hybrid map, a genetic map, and an STS-content YAC map, have been constructed for the chromosome. However, a physical map providing accurate physical distances has not yet been established. A chromosome-wide NotI restriction map was constructed to understand the overall feature of the genome organization and to facilitate the positional cloning of disease genes.
A complete NotI restriction map of the entire long arm of human chromosome 11 was constructed using linking-clone mapping. This physical map covers 77.6Mb, from a pericentromeric NotI site to the terminus, and provides the most accurate ordering and distance estimation to date. We also mapped 138 sequence markers in the q13 region that have been poorly mapped previously.
The restriction map of the entire long arm of human chromosome 11 is the longest restriction map of the human genome. This mapping has disclosed unique features regarding the organization of the chromosome, indicating that restriction sites of NotI, a CpG-recognition enzyme, are primarily distributed in R (or T) bands and that genetic distance is considerably longer in R (or T) bands than in G bands. The mapping, as well as the dense concentration of mapped markers within the q13 region, should help with positional cloning of the genes associated with various diseases.
人类11号染色体是一条常染色体，其上已定位了许多疾病基因。针对该染色体已构建了多种不同类型的图谱，包括辐射杂种图谱、遗传图谱和STS含量酵母人工染色体图谱。然而，尚未建立提供准确物理距离的物理图谱。构建全染色体范围的NotI限制酶切图谱，以了解基因组组织的整体特征并促进疾病基因的定位克隆。
使用连接克隆图谱构建了人类11号染色体整个长臂的完整NotI限制酶切图谱。该物理图谱覆盖了从着丝粒NotI位点到末端的77.6Mb区域，提供了迄今为止最准确的排序和距离估计。我们还在先前定位较差的q13区域定位了138个序列标记。
人类11号染色体整个长臂的限制酶切图谱是人类基因组中最长的限制酶切图谱。该图谱揭示了有关染色体组织的独特特征，表明NotI（一种CpG识别酶）的限制酶切位点主要分布在R（或T）带中，并且R（或T）带中的遗传距离比G带中的长得多。该图谱以及q13区域内定位标记的密集分布，应有助于与各种疾病相关基因的定位克隆。
