Higgins M J, Smilinich N J, Sait S, Koenig A, Pongratz J, Gessler M, Richard C W, James M R, Sanford J P, Kim B W
Department of Human Genetics, Roswell Park Cancer Institute, Buffalo, New York 14263.
Genomics. 1994 Sep 1;23(1):211-22. doi: 10.1006/geno.1994.1479.
An ordered NotI fragment map containing over 60 loci and encompassing approximately 17 Mb has been constructed for human chromosome band 11p15. Forty-two probes, including 11 NotI-linking cosmids, were subregionally mapped to 11p15 using a subset of the J1-deletion hybrids. These and 23 other probes defining loci previously mapped to 11p15 were hybridized to genomic DNA digested with NotI and 5 other infrequently cleaving restriction enzymes and separated by pulsed-field gel electrophoresis. Thirty-nine distinct NotI fragments were detected encompassing approximately 85% of the estimated length of 11p15. The predicted order of the gene loci used is cen-MYOD1-PTH-CALCA-ST5-RBTN1-HPX-HBB-RRM1 -TH/INS/IGF2-H19-CTSD-MUC2-DRD4-HRAS - RNH-tel. This map will allow higher resolution mapping of new 11p15 markers, facilitate positional cloning of disease genes, and provide a framework for the physical mapping of 11p15 in clone contigs.
已构建了一个有序的NotI片段图谱，该图谱包含60多个基因座，覆盖约17兆碱基对，用于人类染色体带11p15。使用J1缺失杂种的一个子集，将包括11个NotI连接黏粒在内的42个探针在区域内定位到11p15。这些探针以及另外23个定义先前已定位到11p15的基因座的探针，与用NotI和其他5种切割频率较低的限制性内切酶消化的基因组DNA杂交，并通过脉冲场凝胶电泳进行分离。检测到39个不同的NotI片段，覆盖了估计的11p15长度的约85%。所使用的基因座的预测顺序是着丝粒-MYOD1-PTH-CALCA-ST5-RBTN1-HPX-HBB-RRM1-TH/INS/IGF2-H19-CTSD-MUC2-DRD4-HRAS-RNH-端粒。该图谱将允许对新的11p15标记进行更高分辨率的定位，促进疾病基因的定位克隆，并为11p15在克隆重叠群中的物理图谱构建提供一个框架。
