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一种用于测量细菌孢子对水蒸气吸附作用的非侵入性方法。

A non-intrusive method for the measurement of water vapour sorption by bacterial spores.

作者信息

Rubel G O

机构信息

Edgewood Research, Development & Engineering Centre, Aberdeen Proving Ground, MD 21010-5423, USA.

出版信息

J Appl Microbiol. 1997 Aug;83(2):243-7. doi: 10.1046/j.1365-2672.1997.00222.x.

DOI:10.1046/j.1365-2672.1997.00222.x
PMID:9281828
Abstract

Single particle levitation (SPL) is used to measure the sorption and desorption of water vapour from microparticles comprising of Bacillus spores. Water gain is determined from increases in the weight-balancing levitation voltages. Spore water isotherms are compared to values previously reported using bulk gravimetric methods. Two salient differences are found between the SPL and bulk data: (1) greater osmotic swelling is observed in the SPL data; and (2) the SPL data exhibit open loop hysteresis while bulk data exhibit closed loop hysteresis.

摘要

单颗粒悬浮(SPL)用于测量芽孢杆菌孢子组成的微粒对水蒸气的吸附和解吸。通过平衡悬浮电压的增加来确定吸水量。将孢子水等温线与先前使用整体重量法报告的值进行比较。在SPL数据和整体数据之间发现了两个显著差异:(1)在SPL数据中观察到更大的渗透膨胀;(2)SPL数据呈现开环滞后,而整体数据呈现闭环滞后。

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