芽孢形成培养基和二价离子对嗜酸嗜热栖热放线菌芽孢耐热性的影响。

Influence of sporulation medium and divalent ions on the heat resistance of Alicyclobacillus acidoterrestris spores.

作者信息

Yamazaki K, Kawai Y, Inoue N, Shinano H

机构信息

Laboratory of Food Wholesomeness, Faculty of Fisheries, Hokkaido University, Hakodate, Japan.

出版信息

Lett Appl Microbiol. 1997 Aug;25(2):153-6. doi: 10.1046/j.1472-765x.1997.00194.x.

DOI:10.1046/j.1472-765x.1997.00194.x

PMID:9281865

Abstract

The influence of divalent cations on the heat resistance of spores of the thermoacidophilic spoilage bacterium Alicyclobacillus acidoterrestris was studied. The heat resistance of A. acidoterrestris spores was not affected by the presence of the different divalent cations (Ca2+, Mg2+, Ba2+, Mn2+ and Sr2+) in the sporulation medium, and by the demineralization or remineralization. And the Ca and Mn contents in A. acidoterrestris spores were scarcely changed by these treatments. However, the heat resistance of Bacillus subtilis spores was affected with the changes of Ca content in the spores. The Ca contents in A. acidoterrestris spores of the different forms were greater than the DPA content. In contrast, the DPA content in the B. subtilis spores was greater than the Ca content. These findings suggest that the presence of constant amount of Ca-DPA and a stronger binding characteristic of divalent ions, especially Ca and Mn, is reflected in the specific heat resistance of A. acidoterrestris spores.

摘要

研究了二价阳离子对嗜热嗜酸腐败菌嗜酸 Alicyclobacillus acidoterrestris 芽孢耐热性的影响。在芽孢形成培养基中存在不同的二价阳离子（Ca2+、Mg2+、Ba2+、Mn2+ 和 Sr2+）以及去矿质化或再矿质化处理，均不会影响嗜酸 Alicyclobacillus acidoterrestris 芽孢的耐热性。并且，这些处理几乎不会改变嗜酸 Alicyclobacillus acidoterrestris 芽孢中的 Ca 和 Mn 含量。然而，枯草芽孢杆菌芽孢的耐热性会随着芽孢中 Ca 含量的变化而受到影响。不同形态的嗜酸 Alicyclobacillus acidoterrestris 芽孢中的 Ca 含量均高于吡啶二羧酸（DPA）含量。相比之下，枯草芽孢杆菌芽孢中的 DPA 含量高于 Ca 含量。这些发现表明，嗜酸 Alicyclobacillus acidoterrestris 芽孢的特定耐热性体现了恒定含量的 Ca-DPA 的存在以及二价离子，尤其是 Ca 和 Mn 的更强结合特性。