Delie F, Rubas W
Genentech, Inc. South San Francisco, CA 94080, USA.
Crit Rev Ther Drug Carrier Syst. 1997;14(3):221-86.
Caco-2 cell monolayers mimic intestinal absorptive epithelium and represent a very useful tool for studying transepithelial transport. The literature on Caco-2 cells is controversial regarding transepithelial resistance and permeabilities of different marker compounds across monolayers. This paper discusses probable causes for these discrepancies. First, we present the role of culture conditions, such as the nature of the support or the passage number, on cell biology and transport properties. Further, we compare the presence of transport proteins in Caco-2 cells to mammalian intestinal tissue and discuss their implication for drug absorption. We also examine the advantages and disadvantages of systems such as Transwell and side-by-side diffusion chambers. A summary of comparisons between permeabilities across Caco-2 monolayers and mammalian intestinal tissues is provided. We conclude that the origin of Caco-2 cells and the culture conditions are in part responsible for the discrepancies encountered in the literature.
Caco-2细胞单层模拟肠道吸收上皮，是研究跨上皮转运的非常有用的工具。关于Caco-2细胞单层的跨上皮电阻以及不同标记化合物的跨膜渗透率，文献中存在争议。本文讨论了这些差异可能的原因。首先，我们阐述培养条件（如支持物的性质或传代次数）对细胞生物学和转运特性的作用。此外，我们将Caco-2细胞中转运蛋白的存在情况与哺乳动物肠道组织进行比较，并讨论它们对药物吸收的影响。我们还研究了Transwell和并排扩散室等系统的优缺点。提供了Caco-2单层膜与哺乳动物肠道组织渗透率比较的总结。我们得出结论，Caco-2细胞的来源和培养条件部分导致了文献中出现的差异。
