Jehle J A, van der Linden I F, Backhaus H, Vlak J M
Department of Virology, Agricultural University, Wageningen, The Netherlands.
Virus Res. 1997 Aug;50(2):151-7. doi: 10.1016/s0168-1702(97)00066-x.
Recently, we described the isolation and characterisation of the novel lepidopteran transposon TCp3.2, which was found to be inserted into the genome of a spontaneous mutant of the baculovirus Cydia pomonella granulovirus (CpGV). Transposon TCp3.2, which is a member of the Tcl/mariner superfamily, is an apparently defective copy which became stably integrated into the viral genome (Jehle et al., 1997. J. Mol. Evol., in press). In this study, we located, cloned and sequenced a genomic region of 2.5 kb of CpGV which encompasses the insertion site of TCp3.2. The TCp3.2 was inserted at a TA dinucleotide as it is typical for many Tcl/mariner-like transposons. The TA insertion site was localised within a non-translated region downstream of the homologous gene of baculovirus late expression factor 2 (lef-2). Additionally, three other complete open reading frames (ORF35Ra, ORF35Rb, and ORF36L) with unknown functions were identified. Transposon insertion into intergenic regions of viral genomes may contribute to the genotypic variability of baculoviruses without any phenotypic effect.
最近，我们描述了新型鳞翅目转座子TCp3.2的分离和特性，该转座子被发现插入到杆状病毒苹果蠹蛾颗粒体病毒（CpGV）自发突变体的基因组中。转座子TCp3.2是Tcl/水手超家族的成员，是一个明显有缺陷的拷贝，它已稳定地整合到病毒基因组中（Jehle等人，1997年。《分子进化杂志》，即将发表）。在本研究中，我们定位、克隆并测序了CpGV 2.5 kb的基因组区域，该区域包含TCp3.2的插入位点。TCp3.2插入在TA二核苷酸处，这是许多Tcl/水手样转座子的典型特征。TA插入位点位于杆状病毒晚期表达因子2（lef-2）同源基因下游的非翻译区内。此外，还鉴定出另外三个功能未知的完整开放阅读框（ORF35Ra、ORF35Rb和ORF36L）。转座子插入病毒基因组的基因间隔区可能有助于杆状病毒的基因型变异性，而不产生任何表型效应。
