Inouhe M, Sugo E, Tohoyama H, Joho M, Nevins D J
Department of Biology, Faculty of Science, Ehime University, Matsuyama, Japan.
Int J Biol Macromol. 1997 Aug;21(1-2):11-4. doi: 10.1016/s0141-8130(97)00034-2.
Autolytic activities were measured in cell walls prepared from the yeast Saccharomyces exiguus. Walls of yeast cells exhibited higher autolytic activities directed toward glucans at the exponential phase of growth when compared to cells at the stationary phase, while glucanase activities in the soluble extract fraction were higher at the stationary phase when compared to exponential phase, suggesting an important role of cell wall glucanases in growth of the yeast cells. Yeast cell walls also exhibited a substantially high autolytic activity of glycoproteins containing mannose throughout growth. These results illustrate the diverse metabolism related to functions of yeast cell walls.
在从酵母嗜杀酵母制备的细胞壁中测定了自溶活性。与稳定期的细胞相比,酵母细胞的壁在生长指数期表现出更高的针对葡聚糖的自溶活性,而可溶提取物部分中的葡聚糖酶活性在稳定期比指数期更高,这表明细胞壁葡聚糖酶在酵母细胞生长中起重要作用。酵母细胞壁在整个生长过程中还表现出含有甘露糖的糖蛋白的显著高自溶活性。这些结果说明了与酵母细胞壁功能相关的多种代谢。