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来自豌豆荚的富含木糖的果胶。

The xylose-rich pectins from pea hulls.

作者信息

Renard C M, Weightman R M, Thibault J F

机构信息

Institut National de la Recherche Agronomique, Centre de Recherches Agro-alimentaires, Laboratoire de Biochimie et Technologie des Glucides, Nantes, France.

出版信息

Int J Biol Macromol. 1997 Aug;21(1-2):155-62. doi: 10.1016/s0141-8130(97)00055-x.

DOI:10.1016/s0141-8130(97)00055-x
PMID:9283030
Abstract

The hot acid extract of pea hull, HSP, was rich in galacturonic acid, arabinose and xylose. It was fractionated by copper precipitation followed by ion-exchange chromatography. The copper-soluble fraction represented 26% of HSP and was mostly composed of an arabinan with a low degree of branching, some heteroxylans and a glucan, probably starch. The copper-precipitate (74% of HSP) contained pectins and some residual arabinan, xylan, glucan and mannan. One of the pectic fractions was rich in terminal xylose and fucose; it could be partially degraded by endo-polygalacturonase but not by endo-xylanase and seemed to contain xylogalacturonans.

摘要

豌豆壳热酸提取物(HSP)富含半乳糖醛酸、阿拉伯糖和木糖。通过铜沉淀法,然后进行离子交换色谱法对其进行分级分离。铜可溶级分占HSP的26%,主要由低分支度的阿拉伯聚糖、一些杂木聚糖和一种葡聚糖(可能是淀粉)组成。铜沉淀物(占HSP的74%)含有果胶以及一些残留的阿拉伯聚糖、木聚糖、葡聚糖和甘露聚糖。其中一种果胶级分富含末端木糖和岩藻糖;它可被内切聚半乳糖醛酸酶部分降解,但不能被内切木聚糖酶降解,似乎含有木糖半乳糖醛酸聚糖。

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引用本文的文献

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Plant Cell. 2013 Jan;25(1):270-87. doi: 10.1105/tpc.112.107334. Epub 2013 Jan 31.
2
A xylogalacturonan epitope is specifically associated with plant cell detachment.一种木糖半乳糖醛酸聚糖表位与植物细胞脱离密切相关。
Planta. 2004 Feb;218(4):673-81. doi: 10.1007/s00425-003-1147-8. Epub 2003 Nov 15.
3
Pectin: cell biology and prospects for functional analysis.
果胶:细胞生物学与功能分析前景
Plant Mol Biol. 2001 Sep;47(1-2):9-27.