Brett C T, Healy S A, McDonald M S, Macgregor C, Baydoun E A
Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, UK.
Int J Biol Macromol. 1997 Aug;21(1-2):169-73. doi: 10.1016/s0141-8130(97)00057-3.
Glucuronoxylan synthesised in vitro by membrane-bound enzymes from etiolated pea epicotyls was found to bind to isolated cell walls from the same tissue in a pH-dependant manner. The binding was maximum at pH 3.5-4.0, and decreased to zero at pH 6. The bound glucuronoxylan could be dissociated from the cell walls by washing at pH 6, and the binding appeared to be non-covalent. Extraction experiments indicated that the glucuronoxylan was binding to hemicellulose in the cell-wall. The observed binding may be significant in the process of cell-wall assembly in vivo.
由黄化豌豆上胚轴的膜结合酶体外合成的葡糖醛酸木聚糖，被发现以pH依赖的方式与同一组织分离的细胞壁结合。在pH 3.5 - 4.0时结合量最大，在pH 6时降至零。结合的葡糖醛酸木聚糖可通过在pH 6下洗涤从细胞壁上解离，且这种结合似乎是非共价的。提取实验表明，葡糖醛酸木聚糖与细胞壁中的半纤维素结合。观察到的这种结合在体内细胞壁组装过程中可能具有重要意义。
