Baydoun E A, Waldron K W, Brett C T
Department of Biology, American University of Beirut, Lebanon.
Biochem J. 1989 Feb 1;257(3):853-8. doi: 10.1042/bj2570853.
A particulate enzyme preparation from etiolated pea (Pisum sativum) epicotyls was found to incorporate xylose from UDP-D-xylose into beta-(1----4)-xylan. The ability of this xylan to act as an acceptor for incorporation of [14C]glucuronic acid from UDP-D-[14C]glucuronic acid in a subsequent incubation was very limited, even though glucuronic acid incorporation was greatly prolonged when UDP-D-xylose was present in the same incubation as UDP-D-[14C]glucuronic acid. This indicated that glucuronic acid could not be added to preformed xylan. However, the presence of UDP-D-glucuronic acid inhibited incorporation of [14C]xylose from UDP-D-[14C]xylose into beta-(1----4)-xylan, and neither S-adenosylmethionine nor acetyl-CoA stimulated either the xylosyltransferase or the glucuronyltransferase.
研究发现,来自黄化豌豆(Pisum sativum)上胚轴的一种颗粒状酶制剂能够将UDP-D-木糖中的木糖掺入β-(1→4)-木聚糖中。在随后的孵育中,这种木聚糖作为从UDP-D-[14C]葡萄糖醛酸中掺入[14C]葡萄糖醛酸的受体的能力非常有限,尽管当UDP-D-木糖与UDP-D-[14C]葡萄糖醛酸在同一孵育体系中存在时,葡萄糖醛酸的掺入时间大大延长。这表明葡萄糖醛酸不能添加到预先形成的木聚糖中。然而,UDP-D-葡萄糖醛酸的存在抑制了UDP-D-[14C]木糖中[14C]木糖掺入β-(1→4)-木聚糖,并且S-腺苷甲硫氨酸和乙酰辅酶A均未刺激木糖基转移酶或葡萄糖醛酸基转移酶。