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豌豆(Pisum sativum)上胚轴中参与葡糖醛酸木聚糖合成的木糖基转移酶和葡糖醛酸基转移酶的相互作用。

The interaction of xylosyltransferase and glucuronyltransferase involved in glucuronoxylan synthesis in pea (Pisum sativum) epicotyls.

作者信息

Baydoun E A, Waldron K W, Brett C T

机构信息

Department of Biology, American University of Beirut, Lebanon.

出版信息

Biochem J. 1989 Feb 1;257(3):853-8. doi: 10.1042/bj2570853.

Abstract

A particulate enzyme preparation from etiolated pea (Pisum sativum) epicotyls was found to incorporate xylose from UDP-D-xylose into beta-(1----4)-xylan. The ability of this xylan to act as an acceptor for incorporation of [14C]glucuronic acid from UDP-D-[14C]glucuronic acid in a subsequent incubation was very limited, even though glucuronic acid incorporation was greatly prolonged when UDP-D-xylose was present in the same incubation as UDP-D-[14C]glucuronic acid. This indicated that glucuronic acid could not be added to preformed xylan. However, the presence of UDP-D-glucuronic acid inhibited incorporation of [14C]xylose from UDP-D-[14C]xylose into beta-(1----4)-xylan, and neither S-adenosylmethionine nor acetyl-CoA stimulated either the xylosyltransferase or the glucuronyltransferase.

摘要

研究发现,来自黄化豌豆(Pisum sativum)上胚轴的一种颗粒状酶制剂能够将UDP-D-木糖中的木糖掺入β-(1→4)-木聚糖中。在随后的孵育中,这种木聚糖作为从UDP-D-[14C]葡萄糖醛酸中掺入[14C]葡萄糖醛酸的受体的能力非常有限,尽管当UDP-D-木糖与UDP-D-[14C]葡萄糖醛酸在同一孵育体系中存在时,葡萄糖醛酸的掺入时间大大延长。这表明葡萄糖醛酸不能添加到预先形成的木聚糖中。然而,UDP-D-葡萄糖醛酸的存在抑制了UDP-D-[14C]木糖中[14C]木糖掺入β-(1→4)-木聚糖,并且S-腺苷甲硫氨酸和乙酰辅酶A均未刺激木糖基转移酶或葡萄糖醛酸基转移酶。

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