Amiel A, Arbov L, Manor Y, Fejgin M, Elis A, Gaber E, Lishner M
Department of Medicine, Meir Hospital, Kfar Saba, Israel.
Cancer Genet Cytogenet. 1997 Sep;97(2):97-100. doi: 10.1016/s0165-4608(96)00341-x.
Chromosomal aberrations can be detected in 50% of patients with chronic lymphocytic leukemia (CLL). A role for tumor suppressor genes in the genesis of lymphoid tumors has been reported. In B-CLL, p53 gene mutations were found in 10-15% of the patients. We used fluorescence in situ hybridization (FISH) to detect p53 deletion in B-CLL. We also correlated the cytogenetic findings with the clinical course. In situ hybridization to interphase nuclei showed monallelic p53 deletion in 6 of 23 patients (26%). The percentage of cells with one p53 signal ranged from 12 to 100. A statistically significant correlation between p53 deletion and progression of CLL was demonstrated. We conclude that FISH is a sensitive and reliable method to detect deletion of specific genes (i.e., p53) in CLL. The finding of p53 deletion is associated with disease progression.
50%的慢性淋巴细胞白血病（CLL）患者可检测到染色体畸变。有报道称肿瘤抑制基因在淋巴肿瘤的发生中起作用。在B-CLL中，10%-15%的患者发现有p53基因突变。我们使用荧光原位杂交（FISH）检测B-CLL中的p53缺失。我们还将细胞遗传学结果与临床病程相关联。对间期核进行原位杂交显示，23例患者中有6例（26%）存在单等位基因p53缺失。具有一个p53信号的细胞百分比在12%至100%之间。p53缺失与CLL进展之间存在统计学上的显著相关性。我们得出结论，FISH是检测CLL中特定基因（即p53）缺失的一种敏感且可靠的方法。p53缺失的发现与疾病进展相关。
