Gidáli J, Fehér I, Harsányi V, Réti M
National Institute of Haematology, Immunology and Blood Transfusion, Budapest, Hungary.
Haematologia (Budap). 1996;28(1):27-32.
Umbilical cord blood has been suggested as a potential source of haemopoietic stem cells for clinical transplantation. Assay of stroma adherent blast colony forming cells (CFU-BL), as a cell type that may predict marrow repopulation, has already been suggested to predict the outcome of bone marrow transplantation. In the present experiments the frequency of CFU-BL in plastic non adherent mononuclear cell fraction (PNAMNC) of umbilical cord blood was studied. If PNAMNC from cord blood was co-incubated with a pre-established stromal layer from normal bone marrow a strict linear correlation between panned PNAMNC and blast colonies were observed. Frequency of CFU-BL in cord blood was significantly lower than that in bone marrow. In cord blood, CFU-GEMM frequency was found to be comparable to that of bone marrow, CFU-GM and BFU-E frequency was reduced. A good correlation between CFU-BL and BFU-E frequency was found both in cord blood and bone marrow. On the other hand, irrespective of their source, correlation between CFU-BL and CFU-GEMM or CFU-GM was weak.
脐带血已被认为是临床移植中造血干细胞的一个潜在来源。作为一种可能预测骨髓再增殖的细胞类型，对基质黏附母细胞集落形成细胞（CFU-BL）的检测已被建议用于预测骨髓移植的结果。在本实验中，研究了脐带血塑料非黏附单核细胞部分（PNAMNC）中CFU-BL的频率。如果将脐带血的PNAMNC与来自正常骨髓的预先建立的基质层共同孵育，观察到淘选后的PNAMNC与母细胞集落之间存在严格的线性相关性。脐带血中CFU-BL的频率显著低于骨髓中的频率。在脐带血中，发现CFU-GEMM频率与骨髓中的相当，CFU-GM和BFU-E频率降低。在脐带血和骨髓中均发现CFU-BL与BFU-E频率之间有良好的相关性。另一方面，无论其来源如何，CFU-BL与CFU-GEMM或CFU-GM之间的相关性较弱。
