Stoppini L, Parisi L, Oropesa C, Muller D
Department of Pharmacology, Centre Médical Universitaire, Geneva, Switzerland.
Neuroscience. 1997 Oct;80(4):1127-36. doi: 10.1016/s0306-4522(97)00132-2.
We developed a model of lesion of Schaffer collaterals in hippocampal organotypic slice cultures to analyse the capacity for sprouting and functional recovery expressed in young (one week old) and old (four week old) slice cultures. Slice cultures were sectioned at different ages of maturation in two separate half-slices and maintained in co-culture. Functional recovery was assessed by measuring synaptic responses elicited across the lesion seven days after the lesion and sprouting was evaluated by biocytin labeling of the regenerating fibers seen under the same conditions. Sprouting and functional recovery were found to be markedly reduced and delayed in old vs young cultures. Preparation of co-cultures between young CA3 and old CA1 half-slices resulted in a significant reduction in the capacity for sprouting and regeneration of the young CA3 neurons. Conversely, co-cultures prepared between old CA3 and young CA1 half-slices showed a markedly enhanced capacity for sprouting and functional recovery of old CA3 neurons. These results indicate that the age-dependent impairment in sprouting and regeneration expressed in cortical regions can be improved by and depends upon the presence of a favourable environment.
我们建立了海马器官型脑片培养中Schaffer侧支损伤模型，以分析在年轻（1周龄）和年老（4周龄）脑片培养物中表达的发芽和功能恢复能力。脑片培养物在不同成熟年龄被切成两个单独的半脑片，并进行共培养。在损伤后7天，通过测量损伤部位引发的突触反应来评估功能恢复，通过在相同条件下对再生纤维进行生物素标记来评估发芽情况。结果发现，与年轻培养物相比，年老培养物中的发芽和功能恢复明显减少且延迟。年轻CA3和年老CA1半脑片之间的共培养制备导致年轻CA3神经元的发芽和再生能力显著降低。相反，年老CA3和年轻CA1半脑片之间制备的共培养物显示年老CA3神经元的发芽和功能恢复能力明显增强。这些结果表明，皮层区域中表达的与年龄相关发芽和再生损伤可通过有利环境的存在得到改善，并且取决于有利环境的存在。
