Cavallero C, Sarti P, Spagnoli L G
Prog Biochem Pharmacol. 1977;13:88-93.
Tritiated thymidine radioautography was employed to study the effect of glucocorticoids on smooth muscle proliferation in rabbit arteries. In the aorta and pulmonary artery of rabbits with cholesterol atherolsclerosis, labelled cell counts showed that these steroids decrease the deoxyribonucleic acid synthesis in the intimal plaques. The inhibitory effect on thymidine uptake was found to be dose dependent and closely related to the antiinflammatory potency of the steroid tested. In another set of experiments the smooth muscle proliferation occurring in the rabbit carotid artery after disendothelization was studied. In these normolipemic animals the inhibition of DNA synthesis was particularly evident in the medial smooth muscle cell; concomitantly, in steroid-treated animals the myointimal thickening in the denuded area was less prominent. It is concluded that very likely the glucocorticoid effect on the artery is due to a direct interaction of the steroid with smooth muscle cells.
采用氚标记胸腺嘧啶核苷放射自显影术研究糖皮质激素对兔动脉平滑肌增殖的影响。在患有胆固醇性动脉粥样硬化的兔主动脉和肺动脉中，标记细胞计数显示这些类固醇可降低内膜斑块中的脱氧核糖核酸合成。发现对胸腺嘧啶核苷摄取的抑制作用呈剂量依赖性，且与所测试类固醇的抗炎效力密切相关。在另一组实验中，研究了去内皮后兔颈动脉中发生的平滑肌增殖。在这些血脂正常的动物中，DNA合成的抑制在内膜平滑肌细胞中尤为明显；同时，在接受类固醇治疗的动物中，剥脱区域的肌内膜增厚不那么明显。得出的结论是，糖皮质激素对动脉的作用很可能是由于类固醇与平滑肌细胞的直接相互作用。
