Cavallero C, Di Tondo U, Mingazzini P L, Nicosia R, Pericoli M N, Sarti P, Spagnoli L G, Villaschi S
Atherosclerosis. 1976 Nov-Dec;25(2-3):145-52. doi: 10.1016/0021-9150(76)90020-4.
Tritiated thymidine radioautography was employed to study the effect of cortisol and other glucocorticoids on cellular proliferation in the aorta and pulmonary artery of rabbits with cholesterol atherosclerosis. Labelled cell counts showed that glucocorticoids, even after one day and at a relatively low dose, decrease sharply the deoxyribonucleic acid synthesis in the intimal plaques. The hormonal influence on [3H]thymidine uptake seems to be a dose-dependent process. The relative potency of these steroids in inhibiting DNA synthesis in the plaques parallels closely their anti-inflammatory effectiveness. Conversely mineralocorticoids, including aldosterone and deoxycorticosterone, increase the rate of DNA synthesis in the plaques. It is concluded that the antiatherogenic effect of glucocorticoids on cholesterol-fed rabbits may be due, at least partly, to the inhibitory effect of these steroids on the DNA synthesis of the cellular components of the intimal plaques.
采用氚标记胸腺嘧啶核苷放射自显影术，研究皮质醇和其他糖皮质激素对胆固醇性动脉粥样硬化家兔主动脉和肺动脉细胞增殖的影响。标记细胞计数显示，即使在一天后且剂量相对较低时，糖皮质激素也会显著降低内膜斑块中的脱氧核糖核酸合成。激素对[3H]胸腺嘧啶摄取的影响似乎是一个剂量依赖性过程。这些类固醇在抑制斑块中DNA合成方面的相对效力与其抗炎效果密切平行。相反，包括醛固酮和脱氧皮质酮在内的盐皮质激素会增加斑块中DNA合成的速率。得出的结论是，糖皮质激素对喂食胆固醇的家兔的抗动脉粥样硬化作用可能至少部分归因于这些类固醇对内膜斑块细胞成分DNA合成的抑制作用。
