Fields K L
Prog Clin Biol Res. 1977;15:179-90.
Two cell surface antigens on rat neural tumor cells are defined by antisera from mice immunized with a rat glioma cell line, 33B. The Common antigen is on rat brain and embryo, and is strongly expressed on the surface of all, or most, rat glioma and neuroblastoma cell lines and tumors. The other Restricted antigen is not present at detectable levels on normal rat tissues, but is on 33B, and on 11 other rat neural tumors or cell lines developed from such tumors, though many other tumors are negative. These 2 antigens are on cell membrane preparations from cells and tumors, and have been further characterized using a quantitative antigen assay. Both antigens are heat labile, and can be destroyed by digestion with proteolytic enzymes. The Common antigen is 10 times more sensitive than the restricted antigen to pronase digestion. Furthermore, spacially separate sites for the 2 antigens are indicated by blocking experiments with pepsin digested antisera. Attempts to purify these antigens further have been frustrated by loss of antigenic activity upon detergent-induced release from the membrane. The tissue and tumor distributions of recently described mouse and rat surface antigens are reviewed. Many of these antigens are present on both brain and kidney, but not on other tissues, though several are shared with embryonic cells or sperm. Several new antigens have been described which may be neuronal specific.
用大鼠胶质瘤细胞系33B免疫的小鼠抗血清可鉴定大鼠神经肿瘤细胞上的两种细胞表面抗原。共同抗原存在于大鼠脑和胚胎中，在所有或大多数大鼠胶质瘤和神经母细胞瘤细胞系及肿瘤表面强烈表达。另一种受限抗原在正常大鼠组织中检测不到，但存在于33B细胞以及由此类肿瘤衍生的其他11种大鼠神经肿瘤或细胞系中，不过许多其他肿瘤呈阴性。这两种抗原存在于细胞和肿瘤的细胞膜制剂中，并已通过定量抗原测定进行了进一步表征。两种抗原均对热不稳定，可被蛋白水解酶消化破坏。共同抗原对链霉蛋白酶消化的敏感性比受限抗原高10倍。此外，用胃蛋白酶消化的抗血清进行阻断实验表明这两种抗原在空间上是分开的位点。由于用去污剂诱导抗原从膜上释放后抗原活性丧失，进一步纯化这些抗原的尝试受挫。本文综述了最近描述的小鼠和大鼠表面抗原的组织和肿瘤分布情况。这些抗原中的许多存在于脑和肾中，但不存在于其他组织中，不过有几种与胚胎细胞或精子共有。已经描述了几种可能是神经元特异性的新抗原。
