DeBault L E, Henriquez E, Hart M N, Cancilla P A
In Vitro. 1981 Jun;17(6):480-94. doi: 10.1007/BF02633509.
An endothelial cell line has been established from a primary culture of cerebral microvessels isolated from Swiss-Webster mice. The microvessels were isolated by a mechanical dispersion and filtration technique. The cells that emerged from these microvessels, maintained in organoid cultures, proliferated and formed plaques of a single or mixed cell type. The endothelial cell line, designated ME-2, was isolated from one such morphologically homogeneous cell plaque, using both cloning ring techniques and C6 glioma-conditioned medium. An endothelial specific antiserum was made in rabbits and was used immunocytochemically to confirm the cell type of origin of the ME-2 cell line. Not only did the cell type specific antiserum react exclusively with endothelial cells in vivo, but in the brain the antiserum localized preferentially to the luminal membrane of the endothelium. The ME-2 endothelial cells have retained several of their unique properties such as cytomorphology, growth characteristics, and cell type specific surface antigens throughout the life of the line (in one case 40 passages before senescence).
已从瑞士韦伯斯特小鼠分离的脑微血管原代培养物中建立了一种内皮细胞系。微血管通过机械分散和过滤技术分离。从这些微血管中分离出来的细胞，在类器官培养中培养，增殖并形成单一或混合细胞类型的斑块。使用克隆环技术和C6胶质瘤条件培养基，从一个这样形态学上均一的细胞斑块中分离出了命名为ME-2的内皮细胞系。用兔制备了内皮特异性抗血清，并用于免疫细胞化学以确认ME-2细胞系的起源细胞类型。不仅细胞类型特异性抗血清在体内仅与内皮细胞反应，而且在脑中该抗血清优先定位于内皮的腔膜。ME-2内皮细胞在该细胞系的整个生命周期中（在一例中传代40次直至衰老）保留了其一些独特特性，如细胞形态、生长特征和细胞类型特异性表面抗原。
