Kerkhof L, Speck M
Institute of Marine and Coastal Sciences, Rutgers University, New Brunswick, New Jersey 08903-0231, USA.
Mol Mar Biol Biotechnol. 1997 Sep;6(3):260-7.
Ribosomal RNA gene dosage was determined for 20 marine heterotrophic bacteria using short probes (< 600 bp) from the Escherichia coli 16S rRNA gene and Southern blot analysis. All Bacterial strains had between 4 and 10 copies of the 16S rRNA genes in their genomes. This report presents important preliminary data for developing quantitative molecular methods to address population dynamics of marine based of 16S rRNA sequences.
利用来自大肠杆菌16S rRNA基因的短探针（<600 bp）和Southern印迹分析，测定了20株海洋异养细菌的核糖体RNA基因剂量。所有细菌菌株的基因组中16S rRNA基因拷贝数在4到10个之间。本报告提供了重要的初步数据，有助于开发定量分子方法以研究基于16S rRNA序列的海洋种群动态。
