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碳源利用和同工酶分析作为产毒新萨托菌属及其相关菌的分类辅助手段。

Carbon source utilization and isoenzyme analysis as taxonomic aids for toxigenic Neosartorya species and their relatives.

作者信息

Varga J, Rinyu E, Kiss I, Botos B, Kozakiewicz Z

机构信息

Department of Microbiology, Attila József University, Szeged, Hungary.

出版信息

Acta Microbiol Immunol Hung. 1997;44(1):1-11.

PMID:9284973
Abstract

Collection strains representing species belonging to the genus Neosartorya and its relatives from section Fumigati of the genus Aspergillus were compared for some of their phenotypic features. The examination of both the carbon source utilization and isoenzyme patterns provided a useful tool for clustering these strains. Many species (e.g. Neosartorya hiratsukae, N. quadricincta, N. spinosa, N. aurata, N. aureola) could readily be distinguished from other species based on their specific isoenzyme and carbon source utilization spectra. Close relationship was observed between the A. fumigatus and N. fischeri strains. Aspergillus strain FRR 1266, which also revealed distinct mitochondrial DNA and nuclear DNA patterns, and amplified DNA profiles, was the closest relative of the recently described N. pseudofischeri species, and could also be distinguished from the A. fumigatus strains by its specific carbon source utilization patterns. High levels of variability were detected among N. glabra and A. viridinutans strains; most of the strains of Australian origin formed distinct clusters.

摘要

对代表新萨托菌属及其来自曲霉属烟曲霉组的近缘属的菌株进行了一些表型特征比较。对碳源利用和同工酶模式的检测为这些菌株的聚类提供了有用的工具。许多物种(如平冢新萨托菌、四带新萨托菌、棘孢新萨托菌、金色新萨托菌、金黄新萨托菌)基于其特定的同工酶和碳源利用谱很容易与其他物种区分开来。在烟曲霉和费氏新萨托菌菌株之间观察到密切关系。曲霉菌株FRR 1266,其线粒体DNA和核DNA模式以及扩增的DNA图谱也显示出明显差异,是最近描述的假费氏新萨托菌物种的最亲近亲属,并且也可以通过其特定的碳源利用模式与烟曲霉菌株区分开来。在光滑新萨托菌和绿棕曲霉菌株中检测到高度变异性;大多数澳大利亚来源的菌株形成了不同的聚类。

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